Talactac Melbourne, Lee Jong-Soo, Moon Hojin, Chowdhury Mohammed Y E, Kim Chul Joong
College of Veterinary Medicine and Biomedical Sciences, Cavite State University, 4122 Cavite, Philippines ; College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Republic of Korea.
College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Republic of Korea.
Adv Virol. 2014;2014:301386. doi: 10.1155/2014/301386. Epub 2014 Dec 30.
This study demonstrates the capacity of HM-γ-PGA treatment to significantly protect murine macrophage cells (RAW 264.7 cells) against NDV infection. Such protection can be explained by the induction of antiviral state of HM-γ-PGA in RAW 264.7 cells via TLR4-mediated IRF-3, IRF-7, IFN-β, and IFN-related gene induction as shown in time-dependent changes in mRNA expression confirmed by polymerase chain reaction (PCR). Moreover, the present research also showed that HM-γ-PGA can induce proinflammatory cytokine secretion in RAW 264.7 as measured by enzyme-linked immunosorbent assay (ELISA). Therefore, our findings suggest that HM-γ-PGA can be a potential antiviral substance that can inhibit NDV infection through its stimulation of antiviral state on RAW 264.7 cells. These results have been consistent with the previous studies showing that HM-γ-PGA can protect RAW 264.7 cells and mice against influenza infection. However, it should be noted that although murine macrophage cells are susceptible to NDV, they are not the natural host cells of the virus; thus further in vivo and in vitro studies involving chicken and chicken immune cells are needed to fully assess the efficacy and applicability of HM-γ-PGA in the poultry industry.
本研究证明了HM-γ-聚谷氨酸处理能够显著保护小鼠巨噬细胞（RAW 264.7细胞）免受新城疫病毒（NDV）感染。这种保护作用可通过RAW 264.7细胞中HM-γ-聚谷氨酸经Toll样受体4（TLR4）介导的干扰素调节因子3（IRF-3）、干扰素调节因子7（IRF-7）、干扰素-β（IFN-β）以及干扰素相关基因的诱导而形成抗病毒状态来解释，这在通过聚合酶链反应（PCR）确认的mRNA表达的时间依赖性变化中得到了体现。此外，本研究还表明，通过酶联免疫吸附测定（ELISA）检测发现，HM-γ-聚谷氨酸可诱导RAW 264.7细胞分泌促炎细胞因子。因此，我们的研究结果表明，HM-γ-聚谷氨酸可能是一种潜在的抗病毒物质，它可通过刺激RAW 264.7细胞的抗病毒状态来抑制NDV感染。这些结果与之前的研究一致，之前的研究表明HM-γ-聚谷氨酸可保护RAW 264.7细胞和小鼠免受流感感染。然而，应该注意的是，尽管小鼠巨噬细胞对NDV敏感，但它们并非该病毒的天然宿主细胞；因此，需要进一步开展涉及鸡和鸡免疫细胞的体内和体外研究，以全面评估HM-γ-聚谷氨酸在禽类产业中的功效和适用性。
