Dai Rui, Rossello Ricardo, Chen Chun-Chun, Kessler Joeran, Davison Ian, Hochgeschwender Ute, Jarvis Erich D
Department of Neurobiology, Duke University Medical Center and Howard Hughes Medical Institute, P.O. Box 3209, Durham, NC 27710, USA.
Department of Neurobiology, Duke University Medical Center and Howard Hughes Medical Institute, P.O. Box 3209, Durham, NC 27710, USA ; Instituto de Células Madres SUAGM, Sistema Universitario Ana G. Méndez, P.O. Box 21345, San Juan, PR 00928-1345, USA.
Stem Cells Int. 2014;2014:182737. doi: 10.1155/2014/182737. Epub 2014 Dec 9.
Pluripotent stem cells have the potential to become any cell in the adult body, including neurons and glia. Avian stem cells could be used to study questions, like vocal learning, that would be difficult to examine with traditional mouse models. Induced pluripotent stem cells (iPSCs) are differentiated cells that have been reprogrammed to a pluripotent stem cell state, usually using inducing genes or other molecules. We recently succeeded in generating avian iPSC-like cells using mammalian genes, overcoming a limitation in the generation and use of iPSCs in nonmammalian species (Rosselló et al., 2013). However, there were no established optimal cell culture conditions for avian iPSCs to establish long-term cell lines and thus to study neuronal differentiation in vitro. Here we present an efficient method of maintaining chicken iPSC-like cells and for differentiating them into action potential generating neurons.
多能干细胞有潜力分化为成体中的任何细胞，包括神经元和神经胶质细胞。禽类干细胞可用于研究诸如发声学习等问题，而这些问题用传统的小鼠模型很难进行研究。诱导多能干细胞（iPSC）是已被重编程为多能干细胞状态的分化细胞，通常是通过导入诱导基因或其他分子来实现。我们最近利用哺乳动物基因成功生成了类似禽类iPSC的细胞，克服了在非哺乳动物物种中iPSC生成和应用方面的一个限制（Rosselló等人，2013年）。然而，目前还没有适用于禽类iPSC的成熟的最佳细胞培养条件，以建立长期细胞系并进而在体外研究神经元分化。在此，我们展示了一种高效的方法，用于维持类似鸡iPSC的细胞并将其分化为能产生动作电位的神经元。
