Mak Siu-Shan, Alev Cantas, Nagai Hiroki, Wrabel Anna, Matsuoka Yoko, Honda Akira, Sheng Guojun, Ladher Raj K
Laboratory for Sensory Development, RIKEN Center for Developmental Biology, Kobe, Japan.
Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology, Kobe, Japan.
Elife. 2015 Sep 11;4:e07178. doi: 10.7554/eLife.07178.
Innate pluripotency of mouse embryos transits from naive to primed state as the inner cell mass differentiates into epiblast. In vitro, their counterparts are embryonic (ESCs) and epiblast stem cells (EpiSCs), respectively. Activation of the FGF signaling cascade results in mouse ESCs differentiating into mEpiSCs, indicative of its requirement in the shift between these states. However, only mouse ESCs correspond to the naive state; ESCs from other mammals and from chick show primed state characteristics. Thus, the significance of the naive state is unclear. In this study, we use zebra finch as a model for comparative ESC studies. The finch blastoderm has mESC-like properties, while chick blastoderm exhibits EpiSC features. In the absence of FGF signaling, finch cells retained expression of pluripotent markers, which were lost in cells from chick or aged finch epiblasts. Our data suggest that the naive state of pluripotency is evolutionarily conserved among amniotes.
随着小鼠胚胎的内细胞团分化为上胚层，其固有的多能性从原始态转变为始发态。在体外，它们的对应物分别是胚胎干细胞（ESCs）和上胚层干细胞（EpiSCs）。FGF信号级联的激活导致小鼠胚胎干细胞分化为小鼠上胚层干细胞，表明其在这些状态转变中是必需的。然而，只有小鼠胚胎干细胞对应于原始态；来自其他哺乳动物和鸡的胚胎干细胞表现出始发态特征。因此，原始态的意义尚不清楚。在本研究中，我们使用斑胸草雀作为比较胚胎干细胞研究的模型。雀胚盘具有类似小鼠胚胎干细胞的特性，而鸡胚盘表现出上胚层干细胞的特征。在没有FGF信号的情况下，雀细胞保留了多能性标记的表达，而这些标记在来自鸡或老龄雀上胚层的细胞中丢失。我们的数据表明，多能性的原始态在羊膜动物中是进化保守的。
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