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通过胚胎注射和共培养，小鼠胚胎干细胞和诱导多能干细胞对嵌合体的贡献。

Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos.

作者信息

Guo Jitong, Wu Baojiang, Li Shuyu, Bao Siqin, Zhao Lixia, Hu Shuxiang, Sun Wei, Su Jie, Dai Yanfeng, Li Xihe

机构信息

Research Center for Animal Genetic Resources of Mongolia Plateau, Inner Mongolia University, Hohhot 010021, China ; Inner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, China.

Inner Mongolia Saikexing Reproductive Biotechnology Co., Ltd., Helingeer 011517, China.

出版信息

Stem Cells Int. 2014;2014:409021. doi: 10.1155/2014/409021. Epub 2014 Dec 28.

DOI:10.1155/2014/409021
PMID:25610470
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4291195/
Abstract

Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripotent stem cells, respectively. Although a significantly higher chimera rate resulted from blastocyst injection, the highest germline contribution resulted from injection of 8-cell embryos with embryonic stem cells. The fully agouti colored chimeras were generated from both injection and coculture of 8-cell embryos with embryonic stem cells. Additionally, microsatellite DNA screening showed that the fully agouti colored chimeras were fully embryonic stem cell derived mice. Unlike embryonic stem cells, the mouse chimeras were only generated from injection of 8-cell embryos with induced pluripotent stem cells and none of these showed germline transmission. The results indicated that injection of 8-cell embryos is the most efficient method for assessing stem cell pluripotency and generating induced pluripotent stem cell chimeras, embryonic stem cell chimeras with germline transmission, and fully mouse embryonic stem cell derived mice.

摘要

囊胚注射和桑椹胚聚集常用于基于干细胞的嵌合贡献来评估干细胞的多能性。为了评估从干细胞生成嵌合体的方案，将8细胞期小鼠胚胎分别与小鼠胚胎干细胞和诱导多能干细胞进行注射或共培养。尽管囊胚注射产生的嵌合率显著更高，但最高的种系贡献来自于将胚胎干细胞注射到8细胞期胚胎中。完全呈刺豚鼠毛色的嵌合体是通过将8细胞期胚胎与胚胎干细胞进行注射和共培养产生的。此外，微卫星DNA筛选表明，完全呈刺豚鼠毛色的嵌合体是完全由胚胎干细胞衍生而来的小鼠。与胚胎干细胞不同，小鼠嵌合体仅通过将诱导多能干细胞注射到8细胞期胚胎中产生，且这些嵌合体均未显示种系传递。结果表明，注射8细胞期胚胎是评估干细胞多能性以及生成诱导多能干细胞嵌合体、具有种系传递的胚胎干细胞嵌合体和完全由小鼠胚胎干细胞衍生的小鼠的最有效方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/ccb408ccbc0e/SCI2014-409021.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/b44710dc8ec9/SCI2014-409021.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/fe2f4e294a25/SCI2014-409021.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/ebc699d66cbd/SCI2014-409021.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/c7694084e345/SCI2014-409021.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/673c1ffe206d/SCI2014-409021.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/ccb408ccbc0e/SCI2014-409021.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/b44710dc8ec9/SCI2014-409021.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/fe2f4e294a25/SCI2014-409021.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/ebc699d66cbd/SCI2014-409021.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/c7694084e345/SCI2014-409021.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/673c1ffe206d/SCI2014-409021.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89ec/4291195/ccb408ccbc0e/SCI2014-409021.006.jpg

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