Maxwell I H, Harrison G S, Wood W M, Maxwell F
University of Colorado Health Sciences Center.
Biotechniques. 1989 Mar;7(3):276-80.
A head-to-tail trimer of the SV40 Bcl I-Bam H1 DNA fragment, specifying polyadenylation of RNA transcripts, was cloned as a cassette flanked by multiple restriction sites. Insertion of the trimer into several expression vectors efficiently prevented spurious expression of reporter genes resulting from transcriptional initiation in prokaryotic plasmid sequences in transfected mammalian cells.
SV40 Bcl I-Bam H1 DNA片段的头对头三聚体，它指定RNA转录本的聚腺苷酸化，被克隆为一个两侧有多个限制性酶切位点的盒式结构。将该三聚体插入几个表达载体中，能有效防止在转染的哺乳动物细胞中，由于原核质粒序列中的转录起始而导致的报告基因的假表达。
