Vinay S D, Raghu K G, Sood P P
Department of Biosciences, Saurashtra University, Rajkot, India.
J Environ Pathol Toxicol Oncol. 1989 Jul-Aug;9(4):351-9.
Acid and alkaline phosphatase changes in various parts of the central nervous system (olfactory bulbs, cerebral hemispheres, cerebellum, medulla oblongata, and spinal cord) were analyzed during methylmercury chloride (MMC) treatment with a dose of 5 mg/kg/day body weight. The drug was subcutaneously introduced into the animals and the enzymes were analyzed after 2, 7, and 15 days' treatment. One group of animals was treated for seven days and kept without drug for another seven days (withdrawal group). The antagonizing capacities of four chelators, namely, N-acetyl-DL-homocysteine thiolactone (NAHT), D-penicillamine (DPA), glutathione (GSH), and sodium selenite (SEL), were also analyzed in relation to the restoration of enzymes. Study results show a linear inhibition of acid and alkaline phosphatases with increasing duration of MMC treatment. However, the magnitude of enzymatic inhibition is different in different brain areas. After 15 days' treatment, maximum inhibition of acid and alkaline phosphatases was recorded in the spinal cord and cerebellum, respectively. Chelators also exhibited differential recovery of the enzymes in various animal groups, as well as in discrete brain areas. No uniformity in the recovery of the enzymes with chelators was observed. However, study results show that biochemical parameters are good indicators of early recognition of neurotoxicity.
在用剂量为5毫克/千克体重的氯化甲基汞（MMC）治疗期间，分析了中枢神经系统各部位（嗅球、大脑半球、小脑、延髓和脊髓）的酸性和碱性磷酸酶变化。将药物皮下注射给动物，并在治疗2、7和15天后分析酶。一组动物治疗7天，然后停药7天（停药组）。还分析了四种螯合剂，即N-乙酰-DL-高半胱氨酸硫内酯（NAHT）、D-青霉胺（DPA）、谷胱甘肽（GSH）和亚硒酸钠（SEL）对酶恢复的拮抗能力。研究结果表明，随着MMC治疗时间的延长，酸性和碱性磷酸酶呈线性抑制。然而，酶抑制的程度在不同脑区有所不同。治疗15天后，脊髓和小脑中分别记录到酸性和碱性磷酸酶的最大抑制。螯合剂在不同动物组以及不同脑区也表现出酶的不同恢复情况。未观察到螯合剂使酶恢复的一致性。然而，研究结果表明，生化参数是早期识别神经毒性的良好指标。
