Sood P P, Raghu K G, Bapu C, Vijayalakshmi K
Department of Bioscience, Saurashtra University, Rajkot, India.
J Environ Pathol Toxicol Oncol. 1993 Jul-Sep;12(3):149-54.
A daily dose of 1 mg/kg and 10 mg/kg of methylmercury chloride (MMC) was injected to separate sets of rats for 2 days, 7 days, and 15 days. Low and high doses of four MMC antagonists, namely N-acetyl-DL-homocysteine thiolactone (NAHT), glutathione (GSH), D-penicillamine (DPA), and sodium selenite (SEL) were injected intramuscularly (i.m.) to MMC-treated animals after an interval of half an hour. The animals were sacrificed on the 3rd, 8th, and 16th day, respectively. An additional group of rats was treated with MMC for 7 days; thereafter, it was kept free of toxic exposure for 7 days and sacrificed on the 15th day. The brain was dissected and separated into olfactory bulbs, cerebral hemispheres, cerebellum, and medulla oblongata. Each part of the central nervous system (CNS) was studied separately. We found a dose- and duration-related acetylcholinesterase (AChE) inhibition in all CNS areas. The use of antagonists showed different effects in different CNS areas, but no appreciable recovery of AChE activity could be obtained with the doses used.
将每日剂量为1毫克/千克和10毫克/千克的氯化甲基汞（MMC）分别注射给不同组的大鼠，持续2天、7天和15天。在间隔半小时后，将低剂量和高剂量的四种MMC拮抗剂，即N-乙酰-DL-高半胱氨酸硫内酯（NAHT）、谷胱甘肽（GSH）、D-青霉胺（DPA）和亚硒酸钠（SEL）肌肉注射给接受MMC处理的动物。分别在第3天、第8天和第16天对动物实施安乐死。另一组大鼠接受MMC处理7天；此后，使其脱离有毒暴露7天，并在第15天实施安乐死。解剖大脑并分离出嗅球、大脑半球、小脑和延髓。对中枢神经系统（CNS）的每个部分分别进行研究。我们发现在所有中枢神经系统区域均存在与剂量和持续时间相关的乙酰胆碱酯酶（AChE）抑制作用。拮抗剂的使用在不同的中枢神经系统区域显示出不同的效果，但在所使用的剂量下，AChE活性未得到明显恢复。
