Pal Robert
Department of Chemistry, Durham University, South Road, DH1 3LE Durham, UK.
Faraday Discuss. 2015;177:507-15. doi: 10.1039/c4fd00158c.
A new modular super-resolution technique called Phase Modulation Nanoscopy (PhMoNa) has been developed in order to break the optical diffraction barrier in Confocal Laser Scanning Microscopy (LSCM). This technique is based on using spatially modulated illumination intensity, whilst harnessing the fluorophore's non-linear emission response. It allows experimental resolution in both lateral and axial domains to be improved by at least a factor of 2. The work is in its initial phase, but by using a custom built Electro Optical Modulator (EOM) in conjunction with functionalised Ln(III) complexes as probes, a sub-diffraction resolution of ∼60 nm was achieved of selected cellular organelles in long term live cell imaging experiments.
为了突破共聚焦激光扫描显微镜（LSCM）中的光学衍射极限，一种名为相位调制纳米显微镜（PhMoNa）的新型模块化超分辨率技术应运而生。该技术基于空间调制照明强度，并利用荧光团的非线性发射响应。它能使横向和轴向领域的实验分辨率至少提高两倍。这项工作尚处于初始阶段，但通过将定制的电光调制器（EOM）与功能化的镧系（III）配合物作为探针相结合，在长期活细胞成像实验中，选定的细胞器实现了约60纳米的亚衍射分辨率。
