艾纳香残渣乙酸乙酯部位对大鼠佐剂性关节炎的抗关节炎及抗氧化作用
[Anti-arthritic and anti-oxidative effect of ethyl acetate fraction of Blumea balsamlfera residues in rat adjuvant-induced arthritis].
作者信息
Xia Yan, Zuo Jian, Li Xiang, Chen Jian-Wei
出版信息
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(19):3819-23.
OBJECTIVE
To study the effect of ethyl acetate fraction of Blumea balsamifera (BBE) residue on treating rats of adjuvant arthritis (AA) and its mechanism.
METHOD
The rats were immunized with the Freund's complete adjuvant (FCA). After modeling, 28 days' treatment with BBE was performed. During the experimental process, rat mass, toe girth, arthritic index (AI), proliferation of immune organs and pathological section were measured. After treatment, blood samples were collected through fossa orbitalis vein for detection of serum SOD, MDA, GSH, NO, OH*, ALP, AST, ALT, NAG and SA content using colorimetric method and IL-1, IL-6, TNF-α content using ELISA method.
RESULT
Administration with BBE (high dose) could significantly ameliorate joint swelling and arthritis index, effectively inhibit synovial hyperplasia, down-regulate the levels of MDA, NO, OH*, ALP, AST, ALT, NAG, SA, IL-1, IL-6, TNF-α and up-regulate the SOD and GSH levels in serum.
CONCLUSION
The results suggested BBE possesses substantial anti-arthritis and antioxidant activities.
目的
研究艾纳香残渣乙酸乙酯部位对佐剂性关节炎（AA）大鼠的治疗作用及其机制。
方法
用弗氏完全佐剂（FCA）免疫大鼠。造模后，给予艾纳香残渣乙酸乙酯部位治疗28天。实验过程中，测量大鼠体重、足趾周长、关节炎指数（AI）、免疫器官增殖及病理切片。治疗后，通过眶静脉取血，采用比色法检测血清超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽（GSH）、一氧化氮（NO）、羟自由基（OH*）、碱性磷酸酶（ALP）、天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、N-乙酰-β-D-氨基葡萄糖苷酶（NAG）和唾液酸（SA）含量，采用酶联免疫吸附测定（ELISA）法检测白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）含量。
结果
高剂量艾纳香残渣乙酸乙酯部位给药可显著改善关节肿胀和关节炎指数，有效抑制滑膜增生，下调血清中MDA、NO、OH*、ALP、AST、ALT、NAG、SA、IL-1、IL-6、TNF-α水平，上调SOD和GSH水平。
结论
结果表明艾纳香残渣乙酸乙酯部位具有显著的抗关节炎和抗氧化活性。
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