Zuo Jian, Xia Yan, Li Xiang, Chen Jian-wei
College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xian Lin Road, Nanjing, China.
College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xian Lin Road, Nanjing, China.
J Ethnopharmacol. 2014 Apr 28;153(2):352-8. doi: 10.1016/j.jep.2014.02.015. Epub 2014 Feb 26.
Securidaca inappendiculata (SI) is a traditional antirheumatic medicine used in China. The present study was designed to investigate the therapeutic efficacy of dichloromethane fraction of SI (SID) at three different doses on adjuvant induced arthritis (AA) rats.
Arthritis severity was evaluated by arthritic score, body weight loss, paw circumference, histological changes and hyperplasia of lymphatic tissues. Serum samples were collected for estimation of superoxide dismutase (SOD), glutathione (GSH), hydroxy radical (OH·), nitric oxide (NO), malondialdehyde (MDA), N-acetyl glucosaminidase (NAG), sialic acid (SA), alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine transaminase (ALT). The levels of GSH, MDA, NAG and SA in liver were also assessed. The levels of interleukin-1 (IL-1), tumor necrosis factor alpha (TNF-α), monocyte chemotactic protein 1 (MCP-1) and vascular endothelial growth factor (VEGF) were determined using ELISA method. Another portion of blood was used for total and differential leucocyte counts.
Administration with SID (at high dose with 100 mg/kg) significantly ameliorated the AA severity, suggested by the modulatory effects on body weight loss, paw swelling, hyperplasia of lymphatic tissues and synovial membrane, neutrocytosis and lymphocytosis. It also decreased levels of NO, MDA and OH·, restored SOD and GSH levels in serum. The abnormal increased levels of AST, ALT, ALP, NAG and SA significantly were reverted (compared with AA rats, P<0.01). A similar result was observed in livers. Levels of IL-1, TNF-α, MCP-1 and VEGF were reduced dramatically by SID too.
The results suggest SID possesses substantial anti-arthritic activity. The therapeutic efficacy may be due to immumodepressive effects, cytokines regulation, increasing membrane stability and antioxidantive activity.
无柄紫金牛（SI）是中国一种传统的抗风湿药物。本研究旨在调查无柄紫金牛二氯甲烷提取物（SID）三种不同剂量对佐剂性关节炎（AA）大鼠的治疗效果。
通过关节炎评分、体重减轻、爪周长、组织学变化以及淋巴组织增生来评估关节炎严重程度。采集血清样本以测定超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、羟自由基（OH·）、一氧化氮（NO）、丙二醛（MDA）、N-乙酰葡糖胺酶（NAG）、唾液酸（SA）、碱性磷酸酶（ALP）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）。还评估了肝脏中GSH、MDA、NAG和SA的水平。使用酶联免疫吸附测定（ELISA）法测定白细胞介素-1（IL-1）、肿瘤坏死因子α（TNF-α）、单核细胞趋化蛋白1（MCP-1）和血管内皮生长因子（VEGF）的水平。另一部分血液用于白细胞总数和分类计数。
给予高剂量（100mg/kg）的SID可显著改善AA严重程度，这体现在对体重减轻、爪肿胀、淋巴组织和滑膜增生、中性粒细胞增多和淋巴细胞增多的调节作用上。它还降低了血清中NO、MDA和OH·的水平，恢复了SOD和GSH水平。与AA大鼠相比，AST、ALT、ALP、NAG和SA异常升高的水平显著恢复（P<0.01）。在肝脏中也观察到类似结果。SID还显著降低了IL-1、TNF-α、MCP-1和VEGF的水平。
结果表明SID具有显著的抗关节炎活性。其治疗效果可能归因于免疫抑制作用、细胞因子调节、增加膜稳定性和抗氧化活性。
