Liu Zhao, Zhong Ju-Ying, Gao Er-Ning, Yang Hong
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(19):3841-5.
Glycyrrhizin acid and licorice flavonoids are the component of Glycyrrhiza uralensis Fisch root that has been used for various medicinal purposes in traditional oriental medicine for thousands of years. Macrophages as a principal component of immune system play an important role in the initiation, modulation and final activation of immune response against pathogens. In the present study, glycyrrhizin acid and licorice flavonoids was investigated the anti-inflammatory effect on lipopolysaccharide (LPS)-induced macrophage cell line of RAW264.7. Well-grown RAW264.7 cells were collected and randomly divided into the blank control group, the LPS(1 mg x L(-1)) group, the dexamethasone (5 mg x L(-1)) with LPS group, the glycyrrhizin acid (400, 80, 16 mg x L(-1)) with LPS group and the licorice flavonoids (200, 40, 8 mg x L(-1)) with LPS group. RAW264.7 cells were cultured in 24-well plates, pre-incubated for 4 h with different concentrations of dexamethasone, glycyrrhizin acid, or licorice flavonoids. Then cells were stimulated for 20 h with LPS. The supernatant of culture medium was collected from each well and determinated the concentrations of cytokines by means of BioPlex mouse cytokines assay. Compared with the control group, the LPS group could significantly induced relatively high levels of granulocyte colony stimulating factor (G-CSF), granulocyte-macrophage colony stimulating factor( GM-CSF), macrophage inflammatory protein-1 alpha (MIP-1α), macrophage inflammatory protein-1 beta (MIP-1β), regulated upon activation normal T cell expressed and secreted factor (RANTES), tumor necrosis factor alpha ( TNF-α), monocyte chemotactic protein 1 (MCP-1), chemokine (C-X-C motif) ligand 1 (KC), eotaxin, interleukin(IL)-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12 (p40), IL-12 (p70), IL-13, and IL-17 secretion (P < 0.05). The glycyrrhizin acid significantly inhibited IL-1β, IL-3, IL-5, IL-6, IL-10, IL-12 (p40), IL-12 (p70), IL-13, Eotaxin and TNF-α secreted by LPS-stimulated RAW264.7 cells (P < 0.05). The expression levels of IL-6 and Eotaxin were observably decreased in the licorice flavonoids with LPS group (P < 0.05). The data presented here suggested that the glycyrrhizin acid and licorice flavonoids modulate various cytokines secreted by macrophages and were important anti-inflammatory constituent of Licorice.
甘草酸和甘草黄酮是甘草根的成分，在传统东方医学中已被用于各种药用目的达数千年之久。巨噬细胞作为免疫系统的主要组成部分，在针对病原体的免疫反应的启动、调节和最终激活中发挥着重要作用。在本研究中，研究了甘草酸和甘草黄酮对脂多糖（LPS）诱导的RAW264.7巨噬细胞系的抗炎作用。收集生长良好的RAW264.7细胞并随机分为空白对照组、LPS（1mg×L⁻¹）组、地塞米松（5mg×L⁻¹）与LPS组、甘草酸（400、80、16mg×L⁻¹）与LPS组以及甘草黄酮（200、40、8mg×L⁻¹）与LPS组。将RAW264.7细胞培养于24孔板中，用不同浓度的地塞米松、甘草酸或甘草黄酮预孵育4小时。然后用LPS刺激细胞20小时。从每个孔中收集培养基上清液，并通过BioPlex小鼠细胞因子测定法测定细胞因子的浓度。与对照组相比，LPS组可显著诱导相对高水平的粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、巨噬细胞炎性蛋白-1α（MIP-1α）、巨噬细胞炎性蛋白-1β（MIP-1β）、活化正常T细胞表达和分泌因子（RANTES）、肿瘤坏死因子α（TNF-α）、单核细胞趋化蛋白1（MCP-1）、趋化因子（C-X-C基序）配体1（KC）、嗜酸性粒细胞趋化因子、白细胞介素（IL）-1α、IL-1β、IL-3、IL-4、IL-5、IL-6、IL-10、IL-12（p40）、IL-12（p70）、IL-13和IL-17分泌（P<0.05）。甘草酸显著抑制LPS刺激的RAW264.7细胞分泌的IL-1β、IL-3、IL-5、IL-6、IL-10、IL-12（p40）、IL-12（p70）、IL-13、嗜酸性粒细胞趋化因子和TNF-α（P<0.05）。甘草黄酮与LPS组中IL-6和嗜酸性粒细胞趋化因子的表达水平明显降低（P<0.05）。此处呈现的数据表明，甘草酸和甘草黄酮可调节巨噬细胞分泌的各种细胞因子，是甘草重要的抗炎成分。
