Cruz Érika de Paula da, Campos Luana, Pereira Filipi da Silva, Magliano Gabriela Campos, Benites Bernar Monteiro, Arana-Chavez Victor Elias, Ballester Rafael Yagüe, Simões Alyne
Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil; Division of Dental Materials, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo (USP), São Paulo, SP, Brazil.
Photodiagnosis Photodyn Ther. 2015 Jun;12(2):298-309. doi: 10.1016/j.pdpdt.2014.12.007. Epub 2015 Jan 19.
Oral mucositis (OM) is a debilitating side effect of chemotherapy, which can be relieved by phototherapy. Antimicrobial photodynamic therapy (aPDT) may be used for the treatment of OM, when infection is present. However, there are no studies showing that aPDT affects tissue repair process when used in the treatment of lesions caused by OM. This work aims to evaluate the effect of aPDT in healing OM induced by 5-Fluorouracil (5-FU). Two hundred forty-five hamsters were divided into two groups, control (C) and experimental, which were subdivided into 4 subgroups (Ch, ChP, ChL, aPDT). C group received only the vehicle of chemotherapy and anesthesia, whereas all animals of the experimental groups received anesthesia and chemotherapy agent 5-FU to induce OM. Ch group received no OM treatment; ChP group received an application of methylene blue (MB) 0.01%; ChL received irradiation with low-power-laser (LPL-660 nm/120 J /cm(2)/40 mW/4.4 J per point); and aPDT received MB and LPL irradiation. OM Clinical severity were daily assessed by a blinded examiner. The animals were sacrificed after 5, 7 and 10 days of experiment and their oral mucosa were removed for biochemical (enzymatic activity of SOD and catalase) and histological analyzes (light microscopy). After statistical analysis was performed, results showed that aPDT reduced the severity of OM on the tenth day of the experiment, when compared to the initial OM score (p < 0.05), as well as increased keratinization with organized collagen deposition in the lamina propria. In conclusion, aPDT can be safely used in animals with infected OM because it does not affect lesion-repairing processes.
口腔黏膜炎（OM）是化疗的一种使人衰弱的副作用，光疗可以缓解这种症状。当存在感染时，抗菌光动力疗法（aPDT）可用于治疗OM。然而，尚无研究表明aPDT在用于治疗由OM引起的病变时会影响组织修复过程。本研究旨在评估aPDT对5-氟尿嘧啶（5-FU）诱导的OM愈合的影响。将245只仓鼠分为两组，即对照组（C）和实验组，实验组再细分为4个亚组（Ch、ChP、ChL、aPDT）。C组仅接受化疗和麻醉的载体，而实验组的所有动物均接受麻醉和化疗药物5-FU以诱导OM。Ch组未接受OM治疗；ChP组接受0.01%亚甲蓝（MB）的应用；ChL组接受低功率激光（LPL-660 nm/120 J/cm²/40 mW/每点4.4 J）照射；aPDT组接受MB和LPL照射。由一位不知情的检查者每日评估OM的临床严重程度。在实验的第5、7和10天处死动物，并取下它们的口腔黏膜进行生化分析（超氧化物歧化酶和过氧化氢酶的酶活性）和组织学分析（光学显微镜检查）。进行统计分析后，结果显示，与初始OM评分相比，aPDT在实验的第10天降低了OM的严重程度（p<0.05），并且增加了角质化，固有层中有组织化的胶原沉积。总之，aPDT可安全用于患有感染性OM的动物，因为它不会影响病变修复过程。
