大鼠脑毛细血管内皮细胞表达功能性血小板源性生长因子B型受体。
Rat brain capillary endothelial cells express functional PDGF B-type receptors.
作者信息
Smits A, Hermansson M, Nistér M, Karnushina I, Heldin C H, Westermark B, Funa K
机构信息
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
出版信息
Growth Factors. 1989;2(1):1-8. doi: 10.3109/08977198909069076.
Immunohistochemical staining revealed the presence of platelet-derived growth factor (PDGF) B-type receptors on capillaries of normal rat brain. Furthermore, capillary endothelial cells isolated from rat brain and grown in tissue culture bound [125I]PDGF-BB but not [125I]PDGF-AA, suggesting that they expressed B-type, but not A-type, PDGF receptors. PDGF-BB and PDGF-AB, but not PDGF-AA, also stimulated incorporation of [3H]thymidine into these cells. Thus, rat brain capillary endothelial cells have functional B-type receptors, and thereby differ from endothelial cells derived from large blood vessels, that do not express PDGF receptors. Our data suggest a possible role for PDGF-BB as an angiogenic factor.
免疫组织化学染色显示，正常大鼠脑毛细血管上存在血小板源性生长因子（PDGF）B型受体。此外，从大鼠脑分离并在组织培养中生长的毛细血管内皮细胞能结合[125I]PDGF - BB，但不结合[125I]PDGF - AA，这表明它们表达B型而非A型PDGF受体。PDGF - BB和PDGF - AB（而非PDGF - AA）也能刺激这些细胞将[3H]胸腺嘧啶掺入。因此，大鼠脑毛细血管内皮细胞具有功能性B型受体，从而不同于源自大血管且不表达PDGF受体的内皮细胞。我们的数据提示PDGF - BB可能作为一种血管生成因子发挥作用。
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