Bao Xiaofeng, Cao Qiansheng, Xu Yazhou, Gao Yuanxue, Xu Yuan, Nie Xuemei, Zhou Baojing, Pang Tao, Zhu Jing
Department of Biochemical Engineering, Nanjing University of Science & Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094, PR China.
Department of Biochemical Engineering, Nanjing University of Science & Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094, PR China.
Bioorg Med Chem. 2015 Feb 15;23(4):694-702. doi: 10.1016/j.bmc.2014.12.070. Epub 2015 Jan 7.
A new Rhodamine B derivative (RBDPA), namely, N(1)-(2-(3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)ethyl)-N(4),N(4)-bis(pyridin-2-ylmethyl)succinamide, was designed, synthesized and structurally characterized to develop a chemosensor. The studies show that RBDPA exhibits high sensitivity and selectivity toward Al(3+) among many other metal cations in an ethanol/H2O (1:1, v/v, pH=7.2, HEPES buffer, 0.1mM) solution. Fluorescence microscopy experiments further demonstrate that RBDPA can be used as a fluorescent probe to detect Al(3+) in living cells.
设计、合成并对一种新型罗丹明B衍生物（RBDPA），即N(1)-(2-(3',6'-双（二乙氨基）-3-氧代螺[异吲哚啉-1,9'-呫吨]-2-基）乙基)-N(4),N(4)-双（吡啶-2-基甲基）琥珀酰胺进行了结构表征，以开发一种化学传感器。研究表明，在乙醇/H₂O（1:1，v/v，pH = 7.2，HEPES缓冲液，0.1 mM）溶液中，RBDPA对多种其他金属阳离子中的Al(3+)表现出高灵敏度和选择性。荧光显微镜实验进一步证明，RBDPA可用作荧光探针来检测活细胞中的Al(3+)。
