一种新型罗丹明B与二（2-吡啶甲基）胺共轭物的合成、评估及其作为铝离子高灵敏度和高选择性化学传感器的研究及其在活细胞成像中的应用
Synthesis and evaluation of a new Rhodamine B and Di(2-picolyl)amine conjugate as a highly sensitive and selective chemosensor for Al3+ and its application in living-cell imaging.
作者信息
Bao Xiaofeng, Cao Qiansheng, Xu Yazhou, Gao Yuanxue, Xu Yuan, Nie Xuemei, Zhou Baojing, Pang Tao, Zhu Jing
机构信息
Department of Biochemical Engineering, Nanjing University of Science & Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094, PR China.
Department of Biochemical Engineering, Nanjing University of Science & Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094, PR China.
出版信息
Bioorg Med Chem. 2015 Feb 15;23(4):694-702. doi: 10.1016/j.bmc.2014.12.070. Epub 2015 Jan 7.
A new Rhodamine B derivative (RBDPA), namely, N(1)-(2-(3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)ethyl)-N(4),N(4)-bis(pyridin-2-ylmethyl)succinamide, was designed, synthesized and structurally characterized to develop a chemosensor. The studies show that RBDPA exhibits high sensitivity and selectivity toward Al(3+) among many other metal cations in an ethanol/H2O (1:1, v/v, pH=7.2, HEPES buffer, 0.1mM) solution. Fluorescence microscopy experiments further demonstrate that RBDPA can be used as a fluorescent probe to detect Al(3+) in living cells.
设计、合成并对一种新型罗丹明B衍生物（RBDPA），即N(1)-(2-(3',6'-双（二乙氨基）-3-氧代螺[异吲哚啉-1,9'-呫吨]-2-基）乙基)-N(4),N(4)-双（吡啶-2-基甲基）琥珀酰胺进行了结构表征，以开发一种化学传感器。研究表明，在乙醇/H₂O（1:1，v/v，pH = 7.2，HEPES缓冲液，0.1 mM）溶液中，RBDPA对多种其他金属阳离子中的Al(3+)表现出高灵敏度和选择性。荧光显微镜实验进一步证明，RBDPA可用作荧光探针来检测活细胞中的Al(3+)。
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