Huang Qi, Zhang Qingyou, Wang Enze, Zhou Yanmei, Qiao Han, Pang Lanfang, Yu Fang
Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Jan 5;152:70-6. doi: 10.1016/j.saa.2015.07.062. Epub 2015 Jul 14.
In this paper, a new fluorescent probe has been synthesized and applied as "off-on" sensor for the detection of Al(3+) with a high sensitivity and excellent selectivity in aqueous media. The sensor was easily prepared by one step reaction between rhodamine B hydrazide and pyridoxal hydrochloride named RBP. The structure of the sensor has been characterized by nuclear magnetic resonance and electron spray ionization-mass spectrometry. The fluorescence intensity and absorbance for the sensor showed a good linearity with the concentration of Al(3+) in the range of 0-12.5μM and 8-44μM, respectively, with detection limits of 0.23μM and 1.90μM. The sensor RBP was preliminarily applied to the determination of Al(3+) in water samples from the lake of Henan University and tap water with satisfying results. Moreover, it can be used as a bioimaging reagent for imaging of Al(3+) in living cells.
本文合成了一种新型荧光探针，并将其作为“关-开”传感器用于在水性介质中高灵敏度、高选择性地检测Al(3+)。该传感器由罗丹明B酰肼与盐酸吡哆醛通过一步反应轻松制备而成，命名为RBP。通过核磁共振和电喷雾电离质谱对该传感器的结构进行了表征。该传感器的荧光强度和吸光度分别在0-12.5μM和8-44μM范围内与Al(3+)浓度呈现良好的线性关系，检测限分别为0.23μM和1.90μM。传感器RBP初步应用于河南大学湖水和自来水中Al(3+)的测定，结果令人满意。此外，它还可作为生物成像试剂用于活细胞中Al(3+)的成像。
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