College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Evid Based Complement Alternat Med. 2014;2014:426843. doi: 10.1155/2014/426843. Epub 2014 Dec 30.
This research was designed to identify whether Gastrodiae Rhizoma ethanol extract (GREE) enhances pentobarbital-induced sleep via γ-aminobutyric acid- (GABA-) ergic systems and modulated sleep architectures in animals. GREE (25, 50, and 100 mg/kg, p.o.) inhibited locomotor activity in mice, in a dose-dependent manner. GREE not only prolonged total sleep time, but also reduced sleep latency time in pentobarbital (42 mg/kg)-treated mice. Subhypnotic pentobarbital (28 mg/kg, i.p.) also increased the number of total sleeping animals in concomitant administration of GREE. GREE (100 mg/kg) alone reduced the count of sleep-wake cycles in electroencephalogram. Furthermore, GREE increased total sleep time and rapid eye movement (REM) sleep. From the in vitro experiments, GREE increased intracellular chloride level in primary cultured cerebellar granule cells. Protein expressions of glutamine acid decarboxylase (GAD) and GABAA receptors subtypes by western blot were increased. Therefore, our study suggested that GREE enhances pentobarbital-induced sleeping behaviors and increased REM via the activation of GABAA-ergic transmission in rodents.
本研究旨在确定天麻乙醇提取物（GREE）是否通过γ-氨基丁酸（GABA）能系统增强戊巴比妥诱导的睡眠，并调节动物的睡眠结构。GREE（25、50 和 100mg/kg，po）以剂量依赖的方式抑制小鼠的运动活性。GREE 不仅延长了总睡眠时间，还缩短了戊巴比妥（42mg/kg）处理小鼠的睡眠潜伏期。亚催眠剂量的戊巴比妥（28mg/kg，ip）与 GREE 同时给药也增加了总睡眠时间内入睡动物的数量。GREE（100mg/kg）单独给药可减少脑电图中的睡眠-觉醒周期计数。此外，GREE 增加了总睡眠时间和快速眼动（REM）睡眠。从体外实验来看，GREE 增加了原代培养小脑颗粒细胞中的细胞内氯离子水平。通过 Western blot 检测到谷氨酸脱羧酶（GAD）和 GABAA 受体亚型的蛋白表达增加。因此，我们的研究表明，GREE 通过激活啮齿动物的 GABA 能传递，增强戊巴比妥诱导的睡眠行为和 REM。
