School of Life Science and Chemical Engineering, Huaiyin Institute of Technology , Huai'an 223003, P. R. China.
J Agric Food Chem. 2015 Feb 11;63(5):1558-61. doi: 10.1021/jf505296k. Epub 2015 Feb 2.
Biobased ionic liquids with cholinium as the cation and amino acids as the anions, which could be prepared from renewable biomaterials by simple neutralization reactions, have recently been described as promising and green solvents. Herein, they were successfully used as the reaction media for enzyme-mediated transphosphatidylation of phosphatidylcholine with l-serine for phosphatidylserine synthesis for the first time. Our results indicated that the highest phosphatidylserine yield of 86.5% was achieved. Moreover, 75% original activity of the enzyme was maintained after being used for 10 batches. The present work could be considered an alternative enzymatic strategy for preparing phosphatidylserine. Additionally, the excellent results make the biobased ionic liquids more promising candidates for use as environmentally friendly solvents in biocatalysis fields.
以胆碱为阳离子、氨基酸为阴离子的生物基离子液体可通过简单的中和反应从可再生生物材料中制备，最近被描述为有前途的绿色溶剂。在此，它们首次被成功用作酶促磷脂酰胆碱与 l-丝氨酸转磷酸化反应的反应介质，用于合成磷脂酰丝氨酸。我们的结果表明，最高获得了 86.5%的磷脂酰丝氨酸产率。此外，酶在使用 10 批后仍保持 75%的原始活性。本工作可以被认为是一种替代的酶法策略，用于制备磷脂酰丝氨酸。此外，优异的结果使生物基离子液体更有希望成为生物催化领域中环境友好型溶剂的候选者。
