福氏志贺菌SF173的过表达、纯化、结晶及初步X射线晶体学分析
Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of SF173 from Shigella flexneri.
作者信息
Kim Ha-Neul, An Jeong-Gi, Lee Yoo-Sup, Seok Seung-Hyeon, Yoo Hee-Seop, Seo Min-Duk
机构信息
Department of Molecular Science andTechnology, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
College of Pharmacy, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
出版信息
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):54-6. doi: 10.1107/S2053230X14025941.
Abstract
Shigella flexneri is a Gram-negative, anaerobic bacterium in the genus Shigella that can cause diarrhoea in humans. SF173, a hypothetical protein from S. flexneri 5a strain M90T, has been cloned, overexpressed, purified and crystallized as a part of laboratory-scale structural genomics project. The SF173 protein was crystallized using the sitting-drop vapour-diffusion method in the presence of 0.8 M succinic acid pH 7.0 at 293 K. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 1.47 Å resolution and belonged to space group I432, with unit-cell parameters a=b=c=110.245 Å.
摘要
福氏志贺菌是志贺菌属中的一种革兰氏阴性厌氧菌，可导致人类腹泻。SF173是福氏志贺菌5a菌株M90T中的一种假定蛋白，作为实验室规模结构基因组学项目的一部分，已被克隆、过表达、纯化并结晶。SF173蛋白在293K下，于pH 7.0的0.8 M琥珀酸存在下，采用坐滴气相扩散法结晶。初步X射线衍射分析表明，该晶体的衍射分辨率为1.47 Å，属于空间群I432，晶胞参数a = b = c = 110.245 Å。
相似文献
1
Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of SF173 from Shigella flexneri.福氏志贺菌SF173的过表达、纯化、结晶及初步X射线晶体学分析
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):54-6. doi: 10.1107/S2053230X14025941.
2
Purification and preliminary crystallographic studies of CutC, a novel copper homeostasis protein from Shigella flexneri.
Protein Pept Lett. 2005 Nov;12(8):823-6. doi: 10.2174/0929866054864184.
3
Protein expression, crystallization and preliminary X-ray crystallographic analysis of the isolated Shigella flexneri VapC toxin.福氏志贺菌分离株VapC毒素的蛋白质表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul;69(Pt 7):762-5. doi: 10.1107/S1744309113014012. Epub 2013 Jun 28.
4
Crystallization and preliminary X-ray diffraction analysis of the arginine repressor ArgR from Bacillus halodurans.嗜碱芽孢杆菌精氨酸阻遏蛋白ArgR的结晶及初步X射线衍射分析
Acta Crystallogr F Struct Biol Commun. 2015 Mar;71(Pt 3):291-4. doi: 10.1107/S2053230X15000904. Epub 2015 Feb 19.
5
Purification, crystallization and preliminary crystallographic analysis of SMU.1108c protein from Streptococcus mutans.变形链球菌中SMU.1108c蛋白的纯化、结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):76-8. doi: 10.1107/S174430911004457X. Epub 2010 Dec 22.
6
Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle.弗氏志贺菌III型分泌系统针状结构亚基MxiH的表达、纯化、结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt 3):302-5. doi: 10.1107/S1744309106006555. Epub 2006 Feb 28.
7
Overexpression, purification, characterization and preliminary crystallographic study of phosphoglycolate phosphatase from Shigella flexneri 2a strain 301.福氏志贺菌2a菌株301磷酸乙醇酸磷酸酶的过表达、纯化、表征及初步晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jan 1;65(Pt 1):29-33. doi: 10.1107/S1744309108039067. Epub 2008 Dec 25.
8
Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL1038 from Burkholderia pseudomallei.来自类鼻疽伯克霍尔德菌的假定蛋白BPSL1038的结晶及初步晶体学研究
Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1697-700. doi: 10.1107/S2053230X14025278. Epub 2014 Nov 28.
9
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2).天蓝色链霉菌A3(2)中假定蛋白SCO4226的克隆、表达、纯化、结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt 9):847-50. doi: 10.1107/S174430910802575X. Epub 2008 Aug 20.
10
Cloning, purification, crystallization and preliminary X-ray analysis of XC229, a conserved hypothetical protein from Xanthomonas campestris.野油菜黄单胞菌中一种保守的假定蛋白XC229的克隆、纯化、结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt 7):694-6. doi: 10.1107/S1744309105018944. Epub 2005 Jun 30.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Protein expression, crystallization and preliminary X-ray crystallographic analysis of the isolated Shigella flexneri VapC toxin.福氏志贺菌分离株VapC毒素的蛋白质表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul;69(Pt 7):762-5. doi: 10.1107/S1744309113014012. Epub 2013 Jun 28.
3
Genome sequence of Shigella flexneri serotype 5a strain M90T Sm.志贺氏菌 5a 血清型 M90T Sm 的基因组序列
J Bacteriol. 2012 Jun;194(11):3022. doi: 10.1128/JB.00393-12.
4
Shigellosis: an old disease in new clothes?志贺氏菌病：换了新面貌的古老疾病？
PLoS Med. 2006 Sep;3(9):e354. doi: 10.1371/journal.pmed.0030354.
5
Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of the Shigella flexneri type III secretion system.福氏志贺菌III型分泌系统组分IpaD的表达、有限蛋白酶解及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt 9):865-8. doi: 10.1107/S1744309106027047. Epub 2006 Aug 11.
6
Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle.弗氏志贺菌III型分泌系统针状结构亚基MxiH的表达、纯化、结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt 3):302-5. doi: 10.1107/S1744309106006555. Epub 2006 Feb 28.
7
Actin-dependent movement of bacterial pathogens.细菌病原体的肌动蛋白依赖性运动。
Nat Rev Microbiol. 2006 Feb;4(2):91-101. doi: 10.1038/nrmicro1320.
8
Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery.志贺氏菌属（细菌性痢疾的病原体）的基因组动态变化与多样性
Nucleic Acids Res. 2005 Nov 7;33(19):6445-58. doi: 10.1093/nar/gki954. Print 2005.
9
Shigella flexneri infection: pathogenesis and vaccine development.福氏志贺菌感染：发病机制与疫苗研发
FEMS Microbiol Rev. 2004 Feb;28(1):43-58. doi: 10.1016/j.femsre.2003.07.002.
10
Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T.福氏志贺菌2a血清型2457T菌株的全基因组序列及比较基因组学
Infect Immun. 2003 May;71(5):2775-86. doi: 10.1128/IAI.71.5.2775-2786.2003.
Zotero 插件