Shaibullah Sofiyah, Mohd-Sharif Nurhikmah, Ho Kok Lian, Firdaus-Raih Mohd, Nathan Sheila, Mohamed Rahmah, Ng Chyan Leong
Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia.
Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang, Selangor 43400, Malaysia.
Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1697-700. doi: 10.1107/S2053230X14025278. Epub 2014 Nov 28.
Melioidosis is an infectious disease caused by the pathogenic bacterium Burkholderia pseudomallei. Whole-genome sequencing revealed that the B. pseudomallei genome includes 5855 coding DNA sequences (CDSs), of which ∼25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 Å resolution. It belonged to space group C2221, with unit-cell parameters a = 85.36, b = 115.63, c = 46.73 Å. The calculated Matthews coefficient (VM) suggests that there are two molecules per asymmetric unit, with a solvent content of 48.8%.
类鼻疽是由致病性细菌类鼻疽伯克霍尔德菌引起的一种传染病。全基因组测序显示，类鼻疽伯克霍尔德菌基因组包含5855个编码DNA序列（CDS），其中约25%编码假设蛋白。一种与病原体相关的假设蛋白BPSL1038在大肠杆菌中过表达，通过气相扩散法进行纯化和结晶。以甲酸钠作为沉淀剂生长的BPSL1038蛋白晶体衍射分辨率达到1.55 Å。它属于空间群C2221，晶胞参数a = 85.36、b = 115.63、c = 46.73 Å。计算得到的马修斯系数（VM）表明，每个不对称单元中有两个分子，溶剂含量为48.8%。
