用含有尿嘧啶的单链 DNA 接头进行酶切，以有效释放亲和选择的循环肿瘤细胞。
Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.
机构信息
Department of Biomedical Engineering, UNC-Chapel Hill, NC, USA.
出版信息
Chem Commun (Camb). 2015 Feb 21;51(15):3266-9. doi: 10.1039/c4cc09765c.
Abstract
We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.
摘要
我们报告了一种新的策略，可高效（∼90%）地从表面释放经亲和选择的细胞，如循环肿瘤细胞（CTCs），同时保持细胞活力（>85%）。该策略利用单链 DNA 将捕获抗体连接到 CTC 选择装置的表面。DNA 接头包含可被切割的尿嘧啶残基。
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