微流控亲和富集后液体活检标志物的可见光释放。
Visible photorelease of liquid biopsy markers following microfluidic affinity-enrichment.
机构信息
Center of BioModular Multi-Scale Systems, Department of Chemistry, University of Kansas, 1567 Irving Hill Rd., Lawrence, KS 66045, USA.
出版信息
Chem Commun (Camb). 2020 Apr 14;56(29):4098-4101. doi: 10.1039/c9cc09598e. Epub 2020 Mar 12.
Abstract
We detail a heterobifunctional, 7-aminocoumarin photocleavable (PC) linker with unique properties to covalently attach Abs to surfaces and subsequently release them with visible light (400-450 nm). The PC linker allowed rapid (2 min) and efficient (>90%) release of CTCs and EVs without damaging their molecular cargo.
摘要
我们详细介绍了一种具有独特性质的异双功能、7-氨基香豆素光裂解（PC）连接物，可将 Abs 共价连接到表面上，并随后用可见光（400-450nm）将其释放。该 PC 连接物允许 CTCs 和 EVs 快速（2 分钟）且高效（>90%）释放，而不会破坏其分子货物。
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