Faculdade e Escola Técnica Novo Rumo, Belo Horizonte, MG, Brazil.
University of California, California, USA; Department of General Pathology, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
Braz J Otorhinolaryngol. 2015 Mar-Apr;81(2):158-66. doi: 10.1016/j.bjorl.2014.12.003. Epub 2014 Dec 27.
Occurrence of apoptosis and expression of proliferative markers are powerful tools to establish a prognosis in the follow-up of cancer.
To evaluate the growth fraction in papillomas and laryngeal squamous cell carcinomas with three degrees of differentiation through apoptosis and the expression of nucleolus organizer regions.
Retrospective study from which paraffin material was submitted to microtomy and hematoxylin-eosin and silver staining. Stained slides were used to quantify the apoptotic index and the number of nucleolus organizer regions by morphometry.
Apoptosis was significantly more frequent in well differentiated carcinomas and in papillomas, and a higher growth fraction of expressed nucleolus organizer regions and cells that expressed a greater than average number of nucleolus organizer regions were more frequently noted in undifferentiated carcinomas.
Thus, it was possible to verify that a high apoptotic index was associated with a lower chance of tumor differentiation in carcinomas, while a greater number of total nucleolus organizer regions, cells expressing nucleolus organizer regions above average and a higher growth fraction were associated with greater likelihood of abnormal cell proliferation and increased tumor differentiation.
细胞凋亡的发生和增殖标志物的表达是评估癌症随访中预后的有力工具。
通过细胞凋亡和核仁组成区的表达来评估分化程度为 3 级的乳头状瘤和喉鳞状细胞癌的细胞增殖分数。
回顾性研究，对石蜡材料进行切片和苏木精-伊红及银染色。通过形态计量学对染色切片进行细胞凋亡指数和核仁组成区数量的定量。
在高分化癌和乳头状瘤中，细胞凋亡更为常见，而在未分化癌中，核仁组成区表达的生长分数和表达核仁组成区数量高于平均值的细胞数量更高。
因此，我们可以验证高凋亡指数与癌分化程度较低有关，而总核仁组成区数量、表达核仁组成区数量高于平均值的细胞和生长分数较高与异常细胞增殖和肿瘤分化程度增加的可能性更大有关。
