Park Eun Joo, Kim Ji-Yun, Jeong Mi Sook, Park Kui Young, Park Kwan Hee, Lee Min Won, Joo Seong Soo, Seo Seong Jun
College of Medicine, Department of Dermatology, Hallym University College of Medicine, Anyang, South Korea.
Institute of Atopic Dermatitis, Department of Dermatology, Chung-Ang University College of Medicine, Seoul, South Korea.
J Dermatol Sci. 2015 Mar;77(3):166-72. doi: 10.1016/j.jdermsci.2014.12.005. Epub 2015 Jan 3.
Quercetin-3-O-(2″-gallate)-α-l-rhamnopyranoside (QGR) is a new quercetin derivative which is isolated from the leaves of Acer ginnala Maxim, a native plant of Korea. Quercetin has several biological effects including antioxidative, anti-inflammatory, and anti-allergic effects. However, the topical effect of QGR on atopic dermatitis (AD) like skin lesion in NC/Nga mice has not been studied.
To evaluate the anti-inflammatory and anti-allergic effect of QGR in a murine model of atopic dermatitis.
We measured inducible nitric oxide synthase (iNOS) and cyclooxygenase -2(COX-2) level in RAW264.7 cell with QGR treatment. And after induction of AD like skin lesions with Dermatophagoides farina (Df) ointment, mice were treated with QGR and control drugs. Clinical scores, interleukin (IL) 4, 5, and 13, serum IgE, eosinophil levels, iNOS and COX-2 level were evaluated.
Results show that mRNA level of iNOS and COX-2 in vitro were decreased after QGR treatment. Topical QGR markedly decreased the iNOS and COX-2 mRNA expressions in the skin. QGR also significantly suppressed the increase in the level of total plasma IgE and eosinophils. In addition, topical application of QGR down-regulated the expressions of the cytokines, IL-4,5 and 13, which were induced by Df ointment stimulation.
In the present study, we showed that topical application of QGR ameliorated Df-induced AD-like inflammatory responses in NC/Nga mice. These results demonstrate that QGR might be beneficial in the treatment of AD.
槲皮素-3-O-(2″-没食子酸酯)-α-L-鼠李糖苷（QGR）是一种新的槲皮素衍生物，从韩国本土植物茶条槭的叶子中分离得到。槲皮素具有多种生物学效应，包括抗氧化、抗炎和抗过敏作用。然而，QGR对NC/Nga小鼠特应性皮炎（AD）样皮肤损伤的局部作用尚未得到研究。
评估QGR在特应性皮炎小鼠模型中的抗炎和抗过敏作用。
我们用QGR处理RAW264.7细胞，检测诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）水平。在用粉尘螨（Df）软膏诱导AD样皮肤损伤后，用QGR和对照药物处理小鼠。评估临床评分、白细胞介素（IL）4、5和13、血清IgE、嗜酸性粒细胞水平、iNOS和COX-2水平。
结果显示，QGR处理后体外iNOS和COX-2的mRNA水平降低。局部应用QGR显著降低皮肤中iNOS和COX-2的mRNA表达。QGR还显著抑制了总血浆IgE水平和嗜酸性粒细胞的增加。此外，局部应用QGR下调了由Df软膏刺激诱导的细胞因子IL-4、5和13的表达。
在本研究中，我们表明局部应用QGR可改善Df诱导的NC/Nga小鼠AD样炎症反应。这些结果表明QGR可能对AD的治疗有益。
