College of Medicine, Chung-Ang University, Seoul 156-756, South Korea.
Phytother Res. 2010 Jul;24(7):1071-7. doi: 10.1002/ptr.3084.
Increased levels of eosinphils, IgE, IL-4, 5, and 13 and pro-inflammatory factors (COX-2, iNOS) are observed in patients with atopic dermatitis (AD). Taxifolin 3-O-beta-D-glucopyranoside (TAX) from the roots of Rhododendron mucronulatum (RM) was examined to determine whether its immunomodulatory effect was applicable for treating atopic dermatitis.A total of 7 groups of NC/Nga mice with AD were treated by topical application or intraperitoneal injection of TAX for 4 weeks. Follow-up evaluations were done to assess the changes in clinical observations, eosinophil counts, and levels of IgE, cytokines, COX-2 and iNOS.In the clinical observation during the experimental period, TAX treatment significantly reduced the severity of AD-like lesions induced in NC/Nga mice. Eosinophil and IgE levels decreased after treatment of the animals with TAX. TAX may thus be associated with improvement of eosinophil-related allergic diseases. The expression of cytokines (IL-4, 5 and 13) was significantly inhibited in the TAX-treated group, suggesting that TAX might play an immunoregulatory role associated with AD. In RT-PCR, iNOS and COX-2 expression levels were reduced in the TAX-treated group. In western blotting, the expression levels of iNOS and COX-2 were also reduced in the TAX-treated group.These findings suggest that TAX is effective for the treatment of AD by preventing the production of inflammatory cytokines and by reducing skin inflammation.
在特应性皮炎（AD）患者中观察到嗜酸性粒细胞、IgE、IL-4、5 和 13 以及促炎因子（COX-2、iNOS）水平升高。从 Rhododendron mucronulatum（RM）的根部检查了杨梅素 3-O-β-D-吡喃葡萄糖苷（TAX），以确定其免疫调节作用是否适用于治疗特应性皮炎。用 TAX 通过局部应用或腹腔注射治疗 7 组 AD 型 NC/Nga 小鼠 4 周。进行随访评估，以评估临床观察、嗜酸性粒细胞计数以及 IgE、细胞因子、COX-2 和 iNOS 水平的变化。在实验期间的临床观察中，TAX 治疗显着减轻了 NC/Nga 小鼠诱导的 AD 样病变的严重程度。用 TAX 治疗动物后，嗜酸性粒细胞和 IgE 水平下降。因此，TAX 可能与改善嗜酸性粒细胞相关的过敏性疾病有关。细胞因子（IL-4、5 和 13）的表达在 TAX 治疗组中显着受到抑制，表明 TAX 可能发挥与 AD 相关的免疫调节作用。在 RT-PCR 中，TAX 治疗组中 iNOS 和 COX-2 的表达水平降低。在 Western blot 中，TAX 治疗组中 iNOS 和 COX-2 的表达水平也降低。这些发现表明 TAX 通过抑制炎症细胞因子的产生和减少皮肤炎症来有效治疗 AD。
