Lin Juan, Chen Jun, He Jun, Chen Jing, Yan Qingyun, Zhou Jizhong, Xie Ping
Donghu Experimental Station of Lake Ecosystems, State Key Laboratory for Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China; Graduate University of Chinese Academy of Sciences, Beijing 10049, PR China.
Donghu Experimental Station of Lake Ecosystems, State Key Laboratory for Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
Toxicon. 2015 Mar;96:50-6. doi: 10.1016/j.toxicon.2015.01.011. Epub 2015 Jan 21.
The short-term exposure to microcystin-LR (MC-LR, one of the most common and toxic variants generated by toxigenic cyanobacteria) induced gut dysfunction such as generation of reactive oxygen species, cell erosion and deficient intestinal absorption of nutrients. However, till now, little is known about its impact on gut microbial community, which has been considered as necessary metabolic assistant and stresses resistant entities for the host. This study was designed to reveal the shift of microbial functional genes in the gut of rat orally gavaged with MC-LR. GeoChip detected a high diversity of bacterial and fungal genes involved in basic metabolic processes and stress resistance. The results showed that the composition of functional genes was significantly changed in rat gut after one week of exposure to MC-LR, and we found some relatively enriched genes that are involved in carbon degradation including chitin, starch and limonene metabolism, and these genes were mainly derived from fungal and bacterial pathogens. In addition, we found large amounts of significantly enriched genes relevant to degradation of the specific carbon compounds, aromatics. The dysbiosis of bacterial and fungal flora gave an implication of pathogens invasion. The enriched gene functions could be linked to acute gastroenteritis induced by MC-LR.
短期暴露于微囊藻毒素-LR（MC-LR，产毒蓝藻产生的最常见且毒性最强的变体之一）会导致肠道功能障碍，如活性氧的产生、细胞侵蚀以及肠道营养吸收不足。然而，迄今为止，关于其对肠道微生物群落的影响却知之甚少，而肠道微生物群落被认为是宿主必需的代谢辅助者和抗应激实体。本研究旨在揭示经口灌胃MC-LR的大鼠肠道中微生物功能基因的变化。GeoChip检测到参与基本代谢过程和抗应激的细菌和真菌基因具有高度多样性。结果表明，暴露于MC-LR一周后，大鼠肠道中功能基因的组成发生了显著变化，并且我们发现了一些相对富集的基因，这些基因参与包括几丁质、淀粉和柠檬烯代谢在内的碳降解过程，且这些基因主要来源于真菌和细菌病原体。此外，我们发现大量与特定碳化合物——芳烃降解相关的显著富集基因。细菌和真菌菌群的失调意味着病原体的入侵。富集的基因功能可能与MC-LR诱导的急性肠胃炎有关。
