一种用于粟酒裂殖酵母时序衰老高通量分析的方法。
A method for high-throughput analysis of chronological aging in Schizosaccharomyces pombe.
作者信息
Stephan Jessica, Ehrenhofer-Murray Ann E
机构信息
Institut für Biologie, Humboldt-Universität Berlin, Chausseestr. 117, 10115, Berlin, Germany.
出版信息
Methods Mol Biol. 2015;1263:93-101. doi: 10.1007/978-1-4939-2269-7_7.
The measurement of chronological life span (CLS) in Schizosaccharomyces pombe is traditionally performed by plating back aliquots of aging liquid cultures on solid medium and counting the number of colony-forming units (CFU). However, this method is labor and cost intensive and therefore not amenable to high-throughput screening. Here, we describe a simple method for CLS measurement using aging minicultures in microtiter plates and batch plate-back for the determination of culture viability. This assay can be used to screen a large number of strains, conditions, or compounds in parallel for effects on aging.
粟酒裂殖酵母中按时间顺序排列的寿命（CLS）的测量传统上是通过将老化液体培养物的等分试样接种到固体培养基上并计数集落形成单位（CFU）的数量来进行的。然而，这种方法 labor 且成本高昂，因此不适用于高通量筛选。在这里，我们描述了一种使用微孔板中的老化微型培养物和批量回板来测定培养物活力的简单CLS测量方法。该测定法可用于并行筛选大量菌株、条件或化合物对衰老的影响。
需注意，原文中“labor”表述有误，正确的可能是“laborious”（费力的） 。
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