利用芽殖酵母筛选抗老化化合物的新快速方法及鉴定白僵菌素I为一种强效活性化合物。

New rapid screening method for anti-aging compounds using budding yeast and identification of beauveriolide I as a potent active compound.

作者信息

Nakaya Shigeru, Mizuno Saki, Ishigami Hiroki, Yamakawa Yasuhiro, Kawagishi Hirokazu, Ushimaru Takashi

机构信息

Faculty of Science, Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.

出版信息

Biosci Biotechnol Biochem. 2012;76(6):1226-8. doi: 10.1271/bbb.110872. Epub 2012 Jun 7.

DOI:10.1271/bbb.110872

PMID:22790951

Abstract

The chronological lifespan (CLS) of budding yeast is a model for the aging of post-mitotic cells in higher eukaryotes. We report here the development of a new method to assess yeast CLS. The new assay is simple, convenient and labor-saving. We applied this new method to screen natural compounds isolated from mushrooms and discovered beauveriolide I as a potent anti-aging agent.

摘要

芽殖酵母的按时间顺序排列的寿命（CLS）是高等真核生物中后有丝分裂细胞衰老的模型。我们在此报告一种评估酵母CLS的新方法的开发。这种新测定法简单、方便且省力。我们应用这种新方法筛选从蘑菇中分离出的天然化合物，并发现白僵菌素I是一种有效的抗衰老剂。