Rangaraju Sunitha, Solis Gregory M, Petrascheck Michael
Department of Chemical Physiology, The Scripps Research Institute, 10550 N Torrey Pines Rd, Mail drop: MEM 268, La Jolla, CA, 92037, USA.
Methods Mol Biol. 2015;1263:139-55. doi: 10.1007/978-1-4939-2269-7_11.
Chemical compounds, which modulate enzymatic activities or those which induce specific phenotypes of interest, are valuable probes to study biological phenomena, as they allow modulation of enzymatic activities and temporal control of protein action. Here, we describe the methodology to conduct large-scale screens for chemical compounds that induce a desired phenotype in the roundworm Caenorhabditis elegans (C. elegans) using 96- or 384-well microtiter plates.
能够调节酶活性的化合物或那些诱导出感兴趣的特定表型的化合物，是研究生物学现象的有价值的探针，因为它们能够调节酶活性并对蛋白质作用进行时间控制。在此，我们描述了一种使用96孔或384孔微量滴定板，针对能在秀丽隐杆线虫（C. elegans）中诱导出所需表型的化合物进行大规模筛选的方法。
