Mehta R, Narayan K G, Notermans S
Ranchi Veterinary College, Birsa Agricultural University, India.
Int J Food Microbiol. 1989 Aug;9(1):45-50. doi: 10.1016/0168-1605(89)90036-6.
A procedure, which we have termed DOT-ELISA, to detect Clostridium perfringens type A enterotoxin on nitrocellulose paper is described. Seventy eight preparations from 39 cultures of C. perfringens type A were tested simultaneously by this and by Plate-ELISA methods. The results were comparable. DOT-ELISA detected as little as 0.02 micrograms of purified enterotoxin and 0.13 micrograms of enterotoxin in cell-free culture supernatant. As little as 0.02 micrograms purified enterotoxin mixed with human faeces could be detected specifically. The method is simple and does not require an ELISA reader.
本文描述了一种我们称为斑点酶联免疫吸附测定（DOT-ELISA）的方法，用于在硝酸纤维素纸上检测A型产气荚膜梭菌肠毒素。用这种方法和酶联免疫吸附测定平板法（Plate-ELISA）同时检测了来自39株A型产气荚膜梭菌培养物的78份制剂。结果具有可比性。DOT-ELISA能检测到低至0.02微克的纯化肠毒素以及无细胞培养上清液中0.13微克的肠毒素。低至0.02微克与人类粪便混合的纯化肠毒素也能被特异性检测到。该方法简单，不需要酶联免疫吸附测定仪。
