Cudjoe K S, Thorsen L I, Sørensen T, Reseland J, Olsvik O, Granum P E
MATFORSK, Norwegian Food Research Institute, As.
Int J Food Microbiol. 1991 Apr;12(4):313-21. doi: 10.1016/0168-1605(91)90145-f.
A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.
基于免疫磁珠（Dynabeads M - 280）开发了一种简单、快速且灵敏的免疫测定法，用于检测和定量粪便及食品提取物中的A型产气荚膜梭菌肠毒素。该测定法在粪便匀浆和接种的肉类提取物中对肠毒素的检测限为2.5 ng/ml。以纯化的肠毒素为标准品，通过交叉免疫电泳和Western免疫印迹技术证实了其特异性。
