Kukula-Koch Wirginia, Mroczek Tomasz
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki St., 20-093, Lublin, Poland,
Anal Bioanal Chem. 2015 Mar;407(9):2581-9. doi: 10.1007/s00216-015-8468-x. Epub 2015 Jan 25.
A rapid hydrostatic counter-current chromatography-thin-layer chromatography-electrospray-ionization time-of-flight mass spectrometry (CCC-TLC-ESI-TOF-MS) technique was established for use in seeking potent anti-Alzheimer's drugs among the acethylcholinesterase inhibitors in Argemone mexicana L. underground parts, with no need to isolate components in pure form. The dichloromethane extract from the roots of Mexican prickly poppy that was most rich in secondary metabolites was subjected to hydrostatic-CCC-based fractionation in descending mode, using a biphasic system composed of petroleum ether-ethyl acetate-methanol-water at the ratio of 1.5:3:2.1:2 (v/v). The obtained fractions were analyzed in a TLC-based AChE-inhibition "Fast Blue B" test. All active components in the fractions, including berberine, protopine, chelerithrine, sanguinarine, coptisine, palmatine, magnoflorine, and galanthamine, were identified in a direct TLC-HPLC-ESI-TOF-MS assay with high accuracy. This is the first time galanthamine has been reported in the extract of Mexican prickly poppy and the first time it has been identified in any member of the Papaveraceae family, in the significant quantity of 0.77%.
建立了一种快速的液-液逆流色谱-薄层色谱-电喷雾电离飞行时间质谱（CCC-TLC-ESI-TOF-MS）技术，用于在墨西哥蓟罂粟地下部分的乙酰胆碱酯酶抑制剂中寻找有效的抗阿尔茨海默病药物，无需分离纯形式的成分。对富含次生代谢物的墨西哥蓟罂粟根部二氯甲烷提取物，采用石油醚-乙酸乙酯-甲醇-水比例为1.5:3:2.1:2（v/v）的双相系统，以液-液逆流色谱进行下行模式的分级分离。对得到的馏分进行基于薄层色谱的乙酰胆碱酯酶抑制“固蓝B”试验分析。通过直接的薄层色谱-高效液相色谱-电喷雾电离飞行时间质谱分析，高精度鉴定出馏分中的所有活性成分，包括小檗碱、原阿片碱、白屈菜红碱、血根碱、黄连碱、巴马汀、木兰花碱和加兰他敏。这是首次在墨西哥蓟罂粟提取物中报道加兰他敏，也是首次在罂粟科任何成员中鉴定出加兰他敏，含量达0.77%。
