Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, 12 Akademicka St., 20-950 Lublin, Poland.
Department of Pharmacognosy with the Medicinal Plants Garden, Medical University of Lublin, 1, Chodźki St., 20-093 Lublin, Poland.
Int J Mol Sci. 2023 Apr 12;24(8):7166. doi: 10.3390/ijms24087166.
In search of novel potential drug candidates that could be used as treatments or prophylactics for memory impairment, an aporphine alkaloid magnoflorine (MAG) isolated from the root of was proven to exhibit beneficial anti-amnestic properties. Its effects on immunoreactivity to parvalbumin in the mouse hippocampus were assessed together with a study on its safety and concentration in the brain and plasma. For this purpose, four experimental groups were created: the MAG10 group-treated with 10 mg MAG/kg b.w. , the MAG20 group-treated with 20 mg MAG/kg b.w. , the MAG50 group-treated with 50 mg MAG/kg b.w. , and a control group-injected with saline at a volume corresponding to their weight. Our results indicated that the hippocampal fields CA1-CA3 were characterized by an elevated number of parvalbumin-immunoreactive neurons (PV-IR) and nerve fibers in mice at the doses of 10 and 20 mg/kg b.w. (). No significant changes to the levels of IL-1β, IL-6 or TNF-α were observed for the above two doses; however, the administration of 50 mg/kg b.w. caused a statistically significant elevation of IL-6, IL-1beta plasma levels and an insignificant raise in the TNF-alpha value. The HPLC-MS analysis showed that the alkaloid's content in the brain structures in the group treated with 50 mg/kg b.w. did not increase proportionally with the administered dose. The obtained results show that MAG is able to influence the immunoreactivity to PV-IR in hippocampal neurons and might act as a neuroprotective compound.
为了寻找可用于治疗或预防记忆障碍的新型潜在药物候选物，从 的根部分离出的阿朴啡生物碱吗啡碱（MAG）被证明具有有益的抗遗忘特性。评估了其对小鼠海马区钙结合蛋白（parvalbumin）免疫反应的影响，并研究了其在大脑和血浆中的安全性和浓度。为此，创建了四个实验组：MAG10 组-用 10 mg MAG/kg b.w. 治疗，MAG20 组-用 20 mg MAG/kg b.w. 治疗，MAG50 组-用 50 mg MAG/kg b.w. 治疗，对照组-用与体重相对应的 生理盐水注射。我们的结果表明，在 10 和 20 mg/kg b.w. 剂量下，海马区 CA1-CA3 特征为钙结合蛋白（parvalbumin）免疫反应性神经元（PV-IR）和神经纤维数量增加（）。对于上述两个剂量，IL-1β、IL-6 或 TNF-α的水平没有观察到显著变化；然而，给予 50 mg/kg b.w. 会导致 IL-6、IL-1beta 血浆水平的统计学显著升高，TNF-alpha 值的升高不显著。HPLC-MS 分析表明，在接受 50 mg/kg b.w. 治疗的组中，大脑结构中的生物碱含量没有与给药剂量成比例地增加。获得的结果表明，MAG 能够影响海马神经元的 PV-IR 免疫反应性，并可能作为一种神经保护化合物发挥作用。
