Jürgens Gerd, Park Misoon, Richter Sandra, Touihri Sonja, Krause Cornelia, El Kasmi Farid, Mayer Ulrike
*ZMBP, Developmental Genetics, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
†ZMBP, Microscopy, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
Biochem Soc Trans. 2015 Feb;43(1):73-8. doi: 10.1042/BST20140246.
Cytokinesis separates the forming daughter cells. Higher plants have lost the ability to constrict the plasma membrane (PM) in the division plane. Instead, trans-Golgi network (TGN)-derived membrane vesicles are targeted to the centre of the division plane and generate, by homotypic fusion, the partitioning membrane named cell plate (CP). The CP expands in a centrifugal fashion until its margin fuses with the PM at the cortical division site. Mutant screens in Arabidopsis have identified a cytokinesis-specific syntaxin named KNOLLE and an interacting Sec1/Munc18 (SM) protein named KEULE both of which are required for vesicle fusion during cytokinesis. KNOLLE is only made during M-phase, targeted to the division plane and degraded in the vacuole at the end of cytokinesis. Here we address mechanisms of KNOLLE trafficking and interaction of KNOLLE with different soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) partners and with SM-protein KEULE, ensuring membrane fusion in cytokinesis.
胞质分裂使正在形成的子细胞分离。高等植物已失去在分裂平面收缩质膜（PM）的能力。取而代之的是，源自反式高尔基体网络（TGN）的膜泡被靶向至分裂平面的中心，并通过同型融合产生名为细胞板（CP）的分隔膜。细胞板以离心方式扩展，直至其边缘在皮层分裂位点与质膜融合。在拟南芥中进行的突变体筛选鉴定出一种名为KNOLLE的胞质分裂特异性 syntaxin蛋白和一种名为KEULE的相互作用的Sec1/Munc18（SM）蛋白，这两种蛋白在胞质分裂期间的囊泡融合过程中都是必需的。KNOLLE仅在M期产生，靶向至分裂平面，并在胞质分裂结束时在液泡中降解。在这里，我们探讨了KNOLLE运输的机制，以及KNOLLE与不同的可溶性N - 乙基马来酰亚胺敏感因子（NSF）附着蛋白（SNAP）受体（SNARE）伴侣以及与SM蛋白KEULE的相互作用，以确保胞质分裂中的膜融合。
