多病原体实时PCR系统给我的患者带来了更多益处：确实如此。 - Suppr

Multi-pathogen real-time PCR system adds benefit for my patients: yes.

作者信息

Cambau Emmanuelle, Bauer Michael

机构信息

Department of Bacteriology, Lariboisiere Hospital, AP-HP, Inserm UMR1137 IAME, Univ Paris Diderot, Sorbonne Paris Cité, Paris, France,

出版信息

Intensive Care Med. 2015 Mar;41(3):528-30. doi: 10.1007/s00134-014-3639-3. Epub 2015 Jan 27.

DOI:10.1007/s00134-014-3639-3

PMID:25619484

Abstract

摘要

相似文献

Multi-pathogen real-time PCR system adds benefit for my patients: yes.多病原体实时PCR系统给我的患者带来了更多益处：确实如此。

Intensive Care Med. 2015 Mar;41(3):528-30. doi: 10.1007/s00134-014-3639-3. Epub 2015 Jan 27.

Multipathogen real-time PCR system adds benefit for my patients: no.多病原体实时聚合酶链反应系统对我的患者没有额外益处。

Intensive Care Med. 2015 Mar;41(3):531-3. doi: 10.1007/s00134-014-3607-y. Epub 2015 Jan 27.

Routine use of a real-time polymerase chain reaction method for detection of bloodstream infections in neutropaenic patients.常规使用实时聚合酶链反应方法检测中性粒细胞减少症患者的血流感染。

Diagn Microbiol Infect Dis. 2013 Feb;75(2):130-4. doi: 10.1016/j.diagmicrobio.2012.10.012. Epub 2012 Nov 22.

Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood.商用聚合酶链反应试剂对血液中细菌病原体的通用 16S rDNA 实时聚合酶链反应检测的活性和 DNA 污染。

Diagn Microbiol Infect Dis. 2010 Jan;66(1):41-9. doi: 10.1016/j.diagmicrobio.2008.07.011. Epub 2008 Aug 21.

Evaluation of a multiplex OnSpot Primer-Extension PCR assay in the diagnosis of sepsis.一种多重即时引物延伸聚合酶链反应检测法在脓毒症诊断中的评估

J Microbiol Methods. 2016 Jan;120:91-3. doi: 10.1016/j.mimet.2015.12.001. Epub 2015 Dec 2.

Rapid diagnosis of sepsis and bacterial meningitis in children with real-time fluorescent quantitative polymerase chain reaction amplification in the bacterial 16S rRNA gene.利用细菌16S rRNA基因的实时荧光定量聚合酶链反应扩增技术对儿童败血症和细菌性脑膜炎进行快速诊断。

Clin Pediatr (Phila). 2009 Jul;48(6):641-7. doi: 10.1177/0009922809333972. Epub 2009 Apr 30.

Diagnosis of bacteremia in febrile neutropenic episodes in children with cancer: microbiologic and molecular approach.儿童癌症患者中性粒细胞减少性发热期菌血症的诊断：微生物学和分子方法。

Pediatr Infect Dis J. 2011 Nov;30(11):957-61. doi: 10.1097/INF.0b013e31822a37d7.

Procalcitonin better than C-reactive protein, erythrocyte sedimentation rate, and white blood cell count in predicting DNAemia in patients with sepsis.在预测脓毒症患者的菌血症方面，降钙素原比C反应蛋白、红细胞沉降率和白细胞计数更具优势。

Scand J Infect Dis. 2014 Nov;46(11):745-52. doi: 10.3109/00365548.2014.936493. Epub 2014 Sep 8.

[Early detection of methicillin resistance by real-time PCR in staphylococci isolated from blood cultures].[通过实时聚合酶链反应对血培养分离出的葡萄球菌进行耐甲氧西林早期检测]

Mikrobiyol Bul. 2012 Oct;46(4):671-5.

Incremental value of multiplex real-time PCR for the early diagnosis of sepsis in the emergency department.多重实时 PCR 在急诊科早期诊断脓毒症中的增值作用。

Swiss Med Wkly. 2014 Feb 4;144:w13911. doi: 10.4414/smw.2014.13911.

引用本文的文献

Real -time PCR for early microbiological diagnosis: is it time?用于早期微生物诊断的实时聚合酶链反应：时机已到？

Intensive Care Med. 2017 Nov;43(11):1714-1716. doi: 10.1007/s00134-017-4793-1. Epub 2017 May 23.

Performance and economic evaluation of the molecular detection of pathogens for patients with severe infections: the EVAMICA open-label, cluster-randomised, interventional crossover trial.严重感染患者病原体分子检测的性能和经济评估：EVAMICA 开放性、整群随机、干预性交叉试验。

Intensive Care Med. 2017 Nov;43(11):1613-1625. doi: 10.1007/s00134-017-4766-4. Epub 2017 Apr 3.

本文引用的文献

Accuracy of LightCycler(®) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review and meta-analysis.LightCycler(®) SeptiFast 在疑似脓毒症患者血液中检测和鉴定病原体的准确性：系统评价和荟萃分析。

Intensive Care Med. 2015 Jan;41(1):21-33. doi: 10.1007/s00134-014-3553-8. Epub 2014 Nov 22.

Diagnostic accuracy of SeptiFast multi-pathogen real-time PCR in the setting of suspected healthcare-associated bloodstream infection.SepTiFast 多重病原体实时 PCR 在疑似医疗保健相关血流感染中的诊断准确性。

Intensive Care Med. 2015 Jan;41(1):86-93. doi: 10.1007/s00134-014-3551-x. Epub 2014 Nov 19.

Year in review in Intensive Care Medicine 2013: III. Sepsis, infections, respiratory diseases, pediatrics.《2013年重症医学年度回顾：III. 脓毒症、感染、呼吸系统疾病、儿科学》

Intensive Care Med. 2014 Apr;40(4):471-83. doi: 10.1007/s00134-014-3235-6. Epub 2014 Feb 12.

A guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2013 recommendations by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM)(a).临床微生物实验室诊断感染性疾病的应用指南：美国传染病学会（IDSA）和美国微生物学会（ASM）的 2013 年推荐意见(a)。

Clin Infect Dis. 2013 Aug;57(4):e22-e121. doi: 10.1093/cid/cit278. Epub 2013 Jul 10.

Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis - a systemic review and meta-analysis.用于快速检测疑似败血症患者病原体的多重 PCR 系统：系统评价和荟萃分析。

PLoS One. 2013 May 29;8(5):e62323. doi: 10.1371/journal.pone.0062323. Print 2013.

Overall burden of bloodstream infection and nosocomial bloodstream infection in North America and Europe.北美和欧洲血流感染和医院获得性血流感染的总体负担。

Clin Microbiol Infect. 2013 Jun;19(6):501-9. doi: 10.1111/1469-0691.12195. Epub 2013 Mar 8.

Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock, 2012.拯救脓毒症运动：严重脓毒症和脓毒性休克管理国际指南，2012 年。

Intensive Care Med. 2013 Feb;39(2):165-228. doi: 10.1007/s00134-012-2769-8. Epub 2013 Jan 30.

Cost analysis of real-time polymerase chain reaction microbiological diagnosis in patients with septic shock.脓毒性休克患者实时聚合酶链反应微生物诊断的成本分析

Anaesth Intensive Care. 2012 Nov;40(6):958-63. doi: 10.1177/0310057X1204000606.

A new workflow for the microbiological diagnosis of febrile neutropenia in patients with a central venous catheter.一种新的血流感染性发热伴中性粒细胞减少症患者中心静脉导管相关微生物诊断流程。

J Antimicrob Chemother. 2013 Apr;68(4):943-6. doi: 10.1093/jac/dks462. Epub 2012 Nov 21.

The changing epidemiology of bacteraemias in Europe: trends from the European Antimicrobial Resistance Surveillance System.欧洲血流感染的流行病因学变化：欧洲抗菌药物耐药性监测系统的趋势。

Clin Microbiol Infect. 2013 Sep;19(9):860-8. doi: 10.1111/1469-0691.12028. Epub 2012 Oct 8.

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Multi-pathogen real-time PCR system adds benefit for my patients: yes.

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