Haghshenas B, Abdullah N, Nami Y, Radiah D, Rosli R, Yari Khosroushahi A
Institute of Biosciences, University Putra Malaysia, Selangor, Malaysia.
J Appl Microbiol. 2015 Apr;118(4):1048-57. doi: 10.1111/jam.12762.
Investigation on the use of herbal-based biopolymers for probiotic-Lactobacillus plantarum 15HN-encapsulation is presented. The objectives are to enhance its oral delivery, colonic release and survival rate of these probiotic cultures in gastrointestinal environment.
Nine types of herbal-based polymers blend with different concentration of alginate alone or mixed with psyllium and fenugreek was used as candidate for encapsulation matrix by applying a simple extrusion method. All the blend formulations recorded high encapsulation efficiency at value >98%. The survival rate of viable probiotic cells under both low pH and high bile salt conditions was also high with value above 80% in 2% (w/v) alginate, alginate+psyllium (1·5 + 0·5%) blend and alginate+fenugreek (1·5 + 0·5%) blend as compared to other polymer formulations and nonencapsulated cells. Their release occurred after 2 h in colonic condition and sustained until the 12th hour incubation period. A value added prebiotic effect was observed in (1·5 + 0·5%) alginate-psyllium formulation.
The high encapsulation efficiency, high viability of cell in low pH, high bile salt and the sustained release rates of probiotic cells in colonic condition during storage time was also observed for these herbal gel formulations.
Herbal-based biopolymers offer added advantages of being prebiotic towards the enhancement of probiotic bacterial growth in the gastrointestinal environment.
介绍基于草药的生物聚合物用于益生菌植物乳杆菌15HN包封的研究。目标是提高其口服递送、结肠释放以及这些益生菌培养物在胃肠道环境中的存活率。
通过简单的挤压方法，将九种不同浓度的基于草药的聚合物与单独的藻酸盐或与车前草和胡芦巴混合用作包封基质的候选物。所有混合配方的包封效率均高于98%。与其他聚合物配方和未包封的细胞相比，在低pH值和高胆汁盐条件下，活益生菌细胞的存活率也很高，在2%（w/v）藻酸盐、藻酸盐+车前草（1.5 + 0.5%）混合物和藻酸盐+胡芦巴（1.5 + 0.5%）混合物中的存活率高于80%。它们在结肠条件下2小时后开始释放，并持续到培养12小时。在（1.5 + 0.5%）藻酸盐-车前草配方中观察到了增值益生元效应。
这些草药凝胶配方在储存期间也具有高包封效率、在低pH值、高胆汁盐条件下细胞的高活力以及益生菌细胞在结肠条件下的持续释放率。
基于草药的生物聚合物在增强胃肠道环境中益生菌细菌生长方面具有益生元的额外优势。
