Sharma Dharmender, Sangha Gurinder Kaur, Khera Kuldeep Singh
Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India.
Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India.
Pestic Biochem Physiol. 2015 Jan;117:9-18. doi: 10.1016/j.pestbp.2014.09.004. Epub 2014 Nov 11.
Triazophos (TZ), a non-systemic broad spectrum organophosphate (OP), is being extensively used against a wide range of pests in agricultural practices. The present study was carried out to investigate the toxic effects of triazophos (TZ) in female Wistar rats. Three sub-chronic dose levels of TZ corresponding to 1/10th, 1/20th and 1/40th of LD50 were given for 30 days to adult female Wistar rats through oral intubation. During the treatment period estrous cycle was significantly altered. Activity levels of different oxidative stress (OS) parameters viz. catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and lipid peroxidation (LPO) were differentially altered in the ovary of treated rats. Estradiol levels were significantly high while progesterone levels were significantly reduced in plasma of 1/10th and 1/20th of LD50 TZ-treated rats. Histomorphological studies of ovary revealed increased follicular atresia and increased ovarian surface epithelial height in 1/10th and 1/20th of LD50 TZ-treated rats. Enhanced apoptosis and necrosis were also observed in ovarian granulosa cells at dose-dependent manner. Results infer that TZ exposure may lead to the number of pathophysiological conditions in female rats and severity increases at high doses.
三唑磷（TZ）是一种非内吸性广谱有机磷酸酯（OP），在农业生产中被广泛用于防治多种害虫。本研究旨在调查三唑磷（TZ）对雌性Wistar大鼠的毒性作用。通过口服灌胃，将相当于LD50的1/10、1/20和1/40的三个亚慢性剂量水平的TZ给予成年雌性Wistar大鼠30天。在治疗期间，发情周期发生了显著改变。在经处理大鼠的卵巢中，不同氧化应激（OS）参数即过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽-S-转移酶（GST）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GPx）和脂质过氧化（LPO）的活性水平发生了不同程度的改变。在给予相当于LD50的1/10和1/20的TZ处理的大鼠血浆中，雌二醇水平显著升高，而孕酮水平显著降低。卵巢组织形态学研究显示，在给予相当于LD50的1/10和1/20的TZ处理的大鼠中，卵泡闭锁增加，卵巢表面上皮高度增加。在卵巢颗粒细胞中也观察到剂量依赖性的凋亡和坏死增强。结果表明，暴露于TZ可能导致雌性大鼠出现多种病理生理状况，且高剂量时严重程度增加。
