Jensen Mie Birgitte Frid, Engberg Jørgen, Larsson Jonas T, Olsen Katharina E P, Torpdahl Mia
Department of Clinical Microbiology, Slagelse Hospital, Ingemannsvej 18, DK-4200 Slagelse, Denmark; Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen, Denmark.
Department of Clinical Microbiology, Slagelse Hospital, Ingemannsvej 18, DK-4200 Slagelse, Denmark.
J Microbiol Methods. 2015 Mar;110:98-101. doi: 10.1016/j.mimet.2015.01.018. Epub 2015 Jan 22.
Subtyping of Clostridium difficile is crucial for outbreak investigations. An extended multilocus variable-number tandem-repeat analysis (eMLVA) of 14 variable number tandem repeat (VNTR) loci was validated in multiplex format compatible with a routine typing laboratory and showed excellent concordance with tandem repeat sequence typing (TRST) and high discriminatory power.
艰难梭菌的分型对于暴发调查至关重要。对14个可变数目串联重复序列（VNTR）位点进行的扩展多位点可变数目串联重复序列分析（eMLVA）以与常规分型实验室兼容的多重形式得到验证，并且与串联重复序列分型（TRST）显示出极好的一致性以及高鉴别力。
