Li Yufu, Liu Tingting, Zhang Mengyang, Pan Chuanying, Liu Xu, Zhao Haiyu, Lan Xianyong
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Animals (Basel). 2024 Aug 15;14(16):2362. doi: 10.3390/ani14162362.
plays a crucial role in regulating the reproductive system, but there are few reports on the effects of on ovarian in dairy cows. Previous studies have verified the presence of a 67-bp mutation in the promoter region of the gene. Hence, we employed ovarian tissues = 2111) and blood samples ( = 108) from Chinese Holstein cows as experimental samples to examine the association between promoter variants and ovarian traits. We extracted DNA from these samples and conducted genetic typing identification on each sample using advanced techniques like PCR and agarose gel electrophoresis. Consequently, the DD, ID, and II genotypes were discovered. and it has been observed that the mutation frequency of this locus is low in the Chinese Holstein cow. Importantly, the correlational analysis unveiled a significant relationship ( < 0.05) between the weight of ovaries in late estrus and the width of ovaries during the estrus interval with the mutation. Result of the RT-PCR revealed that the ID genotype partially diminished the expression of the gene. The results of this study suggest that the identified variable duplication could serve as a potential genetic marker for enhancing the ovarian traits of Chinese Holstein cows.
在调节生殖系统中起着至关重要的作用，但关于其对奶牛卵巢影响的报道较少。先前的研究已证实该基因启动子区域存在一个67bp的突变。因此，我们采用中国荷斯坦奶牛的卵巢组织（n = 2111）和血液样本（n = 108）作为实验样本，以研究该基因启动子变异与卵巢性状之间的关联。我们从这些样本中提取DNA，并使用PCR和琼脂糖凝胶电泳等先进技术对每个样本进行基因分型鉴定。结果发现了DD、ID和II基因型。并且观察到该位点在中国荷斯坦奶牛中的突变频率较低。重要的是，相关性分析揭示了发情后期卵巢重量和发情间期卵巢宽度与该突变之间存在显著关系（P < 0.05）。RT-PCR结果显示ID基因型部分降低了该基因的表达。本研究结果表明，所鉴定的可变重复可能作为改善中国荷斯坦奶牛卵巢性状的潜在遗传标记。
