Qiao Deliang, Wei Chuanbao, Ke Chunlin, Zeng Xiaoxiong
College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.
Food Funct. 2015 Mar;6(3):869-77. doi: 10.1039/c4fo01121j.
Anti-angiogenic activities of crude Hyriopsis cumingii polysaccharides (HCPS) and its purified fractions (HCPS-1, HCPS-2 and HCPS-3) were evaluated in vivo using the chicken embryo chorioallantoic membrane (CAM) assay. The promoting effects of crude HCPS and its purified fractions on the chemotaxis, proliferation and phagocytosis of peritoneal macrophage were tested by cell model in vitro and cyclophosphamide-induced immuno-suppression animal model in vivo. The results showed that HCPS could significantly suppress the neovascularization of chicken embryo CAM and promote peritoneal macrophage migrating to monocyte chemotactic protein-1 (MCP-1), propagating and devouring sheep red blood cell (SRBC) in a dose-dependent manner. In addition, HCPS-3 showed stronger immunostimulatory activities in vitro than crude HCPS, HCPS-1 and HCPS-2. The beneficial effects of HCPS on the immune system might be, at least in part, attributed to the improvement of chemotaxis, proliferation and phagocytosis of peritoneal macrophage. All these results suggest that HCPS is a potential immunoenhancing and anti-tumor agent.
利用鸡胚绒毛尿囊膜（CAM）试验在体内评估了三角帆蚌粗多糖（HCPS）及其纯化级分（HCPS-1、HCPS-2和HCPS-3）的抗血管生成活性。通过体外细胞模型和体内环磷酰胺诱导的免疫抑制动物模型，测试了粗HCPS及其纯化级分对腹膜巨噬细胞趋化性、增殖和吞噬作用的促进作用。结果表明，HCPS能显著抑制鸡胚CAM的新血管形成，并以剂量依赖的方式促进腹膜巨噬细胞向单核细胞趋化蛋白-1（MCP-1）迁移、增殖和吞噬绵羊红细胞（SRBC）。此外，HCPS-3在体外显示出比粗HCPS、HCPS-1和HCPS-2更强的免疫刺激活性。HCPS对免疫系统的有益作用可能至少部分归因于腹膜巨噬细胞趋化性、增殖和吞噬作用的改善。所有这些结果表明，HCPS是一种潜在的免疫增强和抗肿瘤剂。
