甘露糖修饰的血蓝蛋白增强甲壳动物血细胞对病原体的内吞作用。
Mannose-modified hemocyanin enhances pathogen endocytosis by crustacean hemocytes.
作者信息
Li Jiaxi, Aweya Jude Juventus, Zhao Mingming, Zhao Yongzhen, Lin Zhongyang, Chen Xiuli, Zheng Zhihong, Li Pengfei, Yao Defu, Zhang Yueling
机构信息
Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, China; Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Sciences, Nanning, China.
Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, China; Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada; The Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.
出版信息
J Biol Chem. 2025 May 28;301(7):110304. doi: 10.1016/j.jbc.2025.110304.
In crustaceans, hemolymph plasma contains more than 90% hemocyanin, whereas hemocytes have minimal levels, suggesting a regulated uptake mechanism. Here, we demonstrate that in Penaeus vannamei, hemocytes internalize plasma hemocyanin under normal conditions via phagocytosis, clathrin-mediated endocytosis, and micropinocytosis. This uptake is significantly enhanced during bacterial (Vibrio parahaemolyticus, Vibrio alginolyticus, Staphylococcus aureus, Streptococcus iniae) and viral (White spot syndrome virus) infections or upon stimulation with pathogen-associated molecular patterns. While post-translational modifications (PTMs) such as dephosphorylation, deacetylation, and mannosylation enhance hemocyanin's pathogen-binding affinity, only mannosylation promotes mannose receptor-mediated endocytosis for intracellular clearance, whereas dephosphorylation and deacetylation facilitate extracellular pathogen elimination. These findings reveal that hemocyanin functions beyond oxygen transport, acting as an immune effector that undergoes PTMs to enhance intracellular pathogen clearance.
在甲壳类动物中，血淋巴血浆含有超过90%的血蓝蛋白，而血细胞中的含量极低，这表明存在一种受调控的摄取机制。在此，我们证明，在凡纳滨对虾中，血细胞在正常条件下通过吞噬作用、网格蛋白介导的内吞作用和微胞饮作用内化血浆血蓝蛋白。在细菌（副溶血性弧菌、溶藻弧菌、金黄色葡萄球菌、海豚链球菌）和病毒（白斑综合征病毒）感染期间或用病原体相关分子模式刺激后，这种摄取会显著增强。虽然诸如去磷酸化、去乙酰化和甘露糖基化等翻译后修饰（PTM）增强了血蓝蛋白与病原体的结合亲和力，但只有甘露糖基化促进甘露糖受体介导的内吞作用以进行细胞内清除，而去磷酸化和去乙酰化则有助于细胞外病原体的清除。这些发现表明，血蓝蛋白的功能不仅仅是运输氧气，还作为一种免疫效应物，通过翻译后修饰来增强细胞内病原体的清除。
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