Braun Volkmar, Helbig Stephanie, Patzer Silke I, Pramanik Avijit, Römer Christin
Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tübingen, Germany.
Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tübingen, Germany.
Int J Med Microbiol. 2015 Feb;305(2):238-42. doi: 10.1016/j.ijmm.2014.12.006. Epub 2014 Dec 24.
The paper provides a short overview of three investigated bacterial protein toxins, colicin M (Cma) of Escherichia coli, pesticin (Pst) of Yersinia pestis and hemolysin (ShlAB) of Serratia marcescens. Cma and Pst are exceptional among colicins in that they kill bacteria by degrading the murein (peptidoglycan). Both are released into the medium and bind to specific receptor proteins in the outer membrane of sensitive E. coli cells. Subsequently they are translocated into the periplasm by an energy-consuming process using the proton motive force. For transmembrane translocation the colicins unfold and refold in the periplasm. In the case of Cma the FkpA peptidyl prolyl cis-trans isomerase/chaperone is required. ShlA is secreted and activated through ShlB in the outer membrane by a type Vb secretion mechanism.
本文简要概述了三种被研究的细菌蛋白毒素，即大肠杆菌的大肠菌素M（Cma）、鼠疫耶尔森菌的杀鼠菌素（Pst）和粘质沙雷氏菌的溶血素（ShlAB）。Cma和Pst在大肠菌素中较为特殊，它们通过降解胞壁质（肽聚糖）来杀死细菌。两者都释放到培养基中，并与敏感大肠杆菌细胞外膜中的特定受体蛋白结合。随后，它们利用质子动力通过耗能过程转运到周质中。对于跨膜转运，大肠菌素在周质中展开并重新折叠。就Cma而言，需要FkpA肽基脯氨酰顺反异构酶/伴侣蛋白。ShlA通过Vb型分泌机制在外膜中由ShlB分泌并激活。
