通过X波段（9.3GHz）电子顺磁共振波谱法检测紫外线照射对与自由基相互作用的影响。
Effect of UV irradiation on interactions with free radicals examined by an X-band (9.3 GHz) EPR spectroscopy.
作者信息
Ramos Paweł, Pilawa Barbara
机构信息
Department of Biophysics, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia in Katowice, Jedności 8, 41-200 Sosnowiec, Poland.
出版信息
Med Chem Res. 2015;24(2):645-651. doi: 10.1007/s00044-014-1170-2. Epub 2014 Jul 22.
The effect of UVA (315-400 nm) irradiation on interactions with free radicals was examined by the use of electron paramagnetic resonance (EPR) spectroscopy. The changes of antioxidant properties of with time of UV irradiation from 10 to 110 min (10 min steps) were determined. DPPH as the paramagnetic reference was used in this study. Changes of EPR signals of the reference after interactions with nonirradiated and UV-irradiated were detected. Interactions of the tested samples caused decrease of the EPR signal of DPPH as the result of its antioxidant properties. The decrease of the amplitude of EPR line of DPPH was lower for interactions with UV-irradiated . EPR examination confirmed antioxidant properties of . The weaker antioxidant properties of after UV irradiation were pointed out. should be storage in the dark. The tests bring to light usefulness of electron paramagnetic resonance with microwave frequency of 9.3 GHz (an X-band) in examination of storage conditions of pharmacological herbs.
通过电子顺磁共振（EPR）光谱法研究了UVA（315 - 400纳米）照射对与自由基相互作用的影响。测定了紫外线照射10至110分钟（以10分钟为步长）时其抗氧化性能随时间的变化。本研究使用DPPH作为顺磁参考物。检测了参考物与未照射和紫外线照射后的相互作用后EPR信号的变化。由于其抗氧化性能，测试样品的相互作用导致DPPH的EPR信号降低。与紫外线照射后的相互作用中，DPPH的EPR线幅度降低幅度较小。EPR检查证实了其抗氧化性能。指出紫外线照射后其抗氧化性能较弱。应避光保存。这些测试揭示了9.3吉赫兹（X波段）微波频率的电子顺磁共振在药草储存条件检测中的实用性。
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