含蜂胶滴剂和喷雾剂的自由基清除活性——电子顺磁共振波谱检测

Free Radical Scavenging Activity of Drops and Spray Containing Propolis-An EPR Examination.

作者信息

Olczyk Pawel, Komosinska-Vassev Katarzyna, Ramos Pawel, Mencner Lukasz, Olczyk Krystyna, Pilawa Barbara

机构信息

Department of Community Pharmacy, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Kasztanowa 3, 41-200 Sosnowiec, Poland.

Department of Clinical Chemistry and Laboratory Diagnostics, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Jedności 8, 41-200 Sosnowiec, Poland.

出版信息

Molecules. 2017 Jan 13;22(1):128. doi: 10.3390/molecules22010128.

DOI:10.3390/molecules22010128

PMID:28098798

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6155723/

Abstract

The influence of heating at a temperature of 50 °C and UV-irradiation of propolis drops and spray on their free radical scavenging activity was determined. The kinetics of interactions of the propolis samples with DPPH free radicals was analyzed. Interactions of propolis drops and propolis spray with free radicals were examined by electron paramagnetic resonance spectroscopy. A spectrometer generating microwaves of 9.3 GHz frequency was used. The EPR spectra of the model DPPH free radicals were compared with the EPR spectra of DPPH in contact with the tested propolis samples. The antioxidative activity of propolis drops and propolis spray decreased after heating at the temperature of 50 °C. A UV-irradiated sample of propolis drops more weakly scavenged free radicals than an untreated sample. The antioxidative activity of propolis spray increased after UV-irradiation. The sample of propolis drops heated at the temperature of 50 °C quenched free radicals faster than the unheated sample. UV-irradiation weakly changed the kinetics of propolis drops or spray interactions with free radicals. EPR analysis indicated that propolis drops and spray should not be stored at a temperature of 50 °C. Propolis drops should not be exposed to UV-irradiation.

摘要

测定了50°C加热以及蜂胶滴剂和喷雾的紫外线照射对其自由基清除活性的影响。分析了蜂胶样品与DPPH自由基相互作用的动力学。通过电子顺磁共振光谱研究了蜂胶滴剂和蜂胶喷雾与自由基的相互作用。使用了一台产生9.3GHz频率微波的光谱仪。将模型DPPH自由基的电子顺磁共振光谱与与测试的蜂胶样品接触的DPPH的电子顺磁共振光谱进行了比较。50°C加热后，蜂胶滴剂和蜂胶喷雾的抗氧化活性降低。紫外线照射的蜂胶滴剂样品清除自由基的能力比未处理的样品弱。紫外线照射后，蜂胶喷雾的抗氧化活性增加。50°C加热的蜂胶滴剂样品比未加热的样品更快地淬灭自由基。紫外线照射对蜂胶滴剂或喷雾与自由基相互作用的动力学影响较小。电子顺磁共振分析表明，蜂胶滴剂和喷雾不应储存在50°C的温度下。蜂胶滴剂不应暴露在紫外线照射下。