Niimi M
Department of Oral Biology and Oral Pathology, School of Dentistry, University of Otago, Dunedin, New Zealand.
Fungal Genet Biol. 1996 Mar;20(1):79-83. doi: 10.1006/fgbi.1996.0013.
Competence in germ tube formation for exponential phase cells of several Candida albicans was low (less than 14%) in 0.1 M phosphate buffer (pH 6.8) containing 1 mM proline. Addition of 5 mM dibutyryl cyclic AMP (cAMP), a lipophilic cAMP analog, to the induction medium increased germ tube formation up to 50%. This enhancement occurred only when the cells were grown in medium lacking glucose or fructose as a carbon source. N-Acetylglucosamine-induced germ tube formation was enhanced by dibutyryl cAMP under similar conditions, but the enhancement was only slight. Cyclic GMP (cGMP) or AMP did not enhance germ tube formation, while dibutyryl cGMP, theophylline, and butyric acid were inhibitory. These results show that dibutyryl cAMP stimulation of the germ tube formation depends on the growth phase and the nutritional status of the cell.
在含有1 mM脯氨酸的0.1 M磷酸盐缓冲液（pH 6.8）中，几种白色念珠菌指数生长期细胞的芽管形成能力较低（低于14%）。向诱导培养基中添加5 mM二丁酰环磷腺苷（cAMP），一种亲脂性cAMP类似物，可使芽管形成增加至50%。这种增强仅在细胞于缺乏葡萄糖或果糖作为碳源的培养基中生长时发生。在类似条件下，N-乙酰葡糖胺诱导的芽管形成也被二丁酰cAMP增强，但增强作用较小。环鸟苷酸（cGMP）或腺苷酸（AMP）不能增强芽管形成，而二丁酰cGMP、茶碱和丁酸则具有抑制作用。这些结果表明，二丁酰cAMP对芽管形成的刺激取决于细胞的生长阶段和营养状态。
