Jeffries T W, Timourian H, Ward R L
Appl Environ Microbiol. 1978 Apr;35(4):704-10. doi: 10.1128/aem.35.4.704-710.1978.
Anabaena cylindrica sparged with argon gas produced H2 continuously for 30 days under limited light conditions (6.0 W/m2) and for 18 days under elevated light conditions (32 W/m2) in the absence of exogenous nitrogen. The efficiency of converting visible light energy (32 W/m2) into chemical energy that is trapped as H2 ranged between 0.35 and 0.85% (approximately 13 microliter of H2 per mg [drywt] per h). Ammonium additions (0.2 mM NH4+) at various times destabilized the system and eventually suppressed H2 production completely, as compared with the control. Cultures grown with 5.0 mg of Fe3+ per liter produced H2 at a rate about twice that of cultures with 0.5 mg of Fe3+ per liter. Cultures grown at pH 7.4 produced H2 at the same initial rates as cultures that were grown at pH 9.4; however, the latter cultures continued to produce H2 after CO2 deprivation.
在无外源氮的情况下，用氩气鼓泡的圆柱鱼腥藻在有限光照条件（6.0瓦/平方米）下持续产生氢气30天，在增强光照条件（32瓦/平方米）下持续产生氢气18天。将可见光能量（32瓦/平方米）转化为以氢气形式捕获的化学能的效率在0.35%至0.85%之间（约每毫克[干重]每小时产生13微升氢气）。与对照相比，在不同时间添加铵（0.2毫摩尔NH4+）会使系统不稳定，并最终完全抑制氢气产生。每升添加5.0毫克Fe3+培养的藻培养物产生氢气的速率约为每升添加0.5毫克Fe3+培养的藻培养物的两倍。在pH 7.4条件下培养的藻培养物与在pH 9.4条件下培养的藻培养物初始产生氢气的速率相同；然而，后者在二氧化碳剥夺后仍继续产生氢气。
