Weissman J C, Benemann J R
Appl Environ Microbiol. 1977 Jan;33(1):123-31. doi: 10.1128/aem.33.1.123-131.1977.
Nitrogen-starved cultures of the alga Anabaena cylindrica 629 produced hydrogen and oxygen continuously for 7 to 19 days. Hydrogen production attained a maximum level after 1 to 2 days of starvation and was followed by a slow decline. The maximum rates were 30 ml of H2 evolved per liter of culture per h or 32 mul of H2 per mg of dry weight per h. In 5 to 7 days the rate of H2 evolution by the more productive cultures fell to one-half its maximum value. The addition of 10(-4) to 5 X 10(-4) M ammonium increased the rate of oxygen evolution and the total hydrogen production of the cultures. H2-O2 ratios were 4:1 under conditions of complete nitrogen starvation and about 1.7:1 after the addition of ammonium. Thus, oxygen evolution was affected by the extent of the nitrogen starvation. Thermodynamic efficiencies of converting incident light energy to free energy of hydrogen via algal photosynthesis were 0.4%. Possible factors limiting hydrogen production were decline of reductant supply and filament breakage. Hydrogen production by filamentous, heterocystous blue-green algae could be used for development of a biophotolysis system.
藻青菌圆柱鱼腥藻629的缺氮培养物持续7至19天产生氢气和氧气。饥饿1至2天后产氢达到最高水平，随后缓慢下降。最高产氢速率为每升培养物每小时产生30毫升氢气，或每毫克干重每小时产生32微升氢气。在5至7天内，高产培养物的氢气释放速率降至其最大值的一半。添加10⁻⁴至5×10⁻⁴M的铵会提高培养物的氧气释放速率和总产氢量。在完全缺氮条件下，氢气与氧气的比例为4:1，添加铵后约为1.7:1。因此，氧气释放受缺氮程度的影响。通过藻类光合作用将入射光能转化为氢气自由能的热力学效率为0.4%。限制产氢的可能因素是还原剂供应减少和丝状藻丝断裂。丝状、具异形胞的蓝藻产氢可用于生物光解系统的开发。
