J Invest Dermatol. 1966 Aug;47(1):16-21.
The sterol and alcohols of human hair and scalp lipids have been concentrated by saponification and chromatography of the unsaponifiable portion over alumina; the alcoholic components (Fraction V) were eluted from the column by means of absolute methanol. For one large batch of lipids (CF-45E), Fraction V was filtered from solid and the resulting rectified oil dissolved in n-hexane, chromatographed over partially spent silica gel (activity: 20) and the column eluted with benzene, chloroform, 1-butanol and methanol. By this method, separation of high and low-molecular weight components and polar materials was afforded. The alcohols were converted to the acetates and analyzed by gas chromatography. A proximate analysis of sterol and alcohols is summarized in Table V. The distribution of fatty alcohols in Fractions V from lipids collected according to sex, race and scalp condition was rather consistent from pool to pool and no unequivocal differences could be attributed to any particular category though a few discrepancies were apparent. Thus, an irregularity was noted in the C20 component from two pools and branched C14 and C16 alcohols could not be detected in yet another one.
人的头发和头皮脂质中的甾醇和醇类，经皂化作用和氧化铝上非皂化物部分的色谱分离已被浓缩；醇类成分（V 馏分）通过用甲醇将柱洗脱。对于一批较大的脂质（CF-45E），从固体中过滤出 V 馏分，所得精制油溶解于正己烷中，用部分失活硅胶（活性：20）进行色谱分离，用苯、氯仿、1-丁醇和甲醇洗脱柱。通过这种方法，可以实现高分子量和低分子量成分以及极性物质的分离。将醇类转化为醋酸盐并通过气相色谱进行分析。甾醇和醇的近似分析总结在表 V 中。根据性别、种族和头皮状况收集的脂质中 V 馏分的脂肪酸醇分布相当一致，尽管存在一些差异，但没有任何明确的差异可归因于任何特定类别。因此，从两个池中注意到 C20 组分的不规则性，而在另一个池中则无法检测到支链 C14 和 C16 醇。
