O'Neill H J, Gershbein L L
J Chromatogr Sci. 1976 Jan;14(1):28-36. doi: 10.1093/chromsci/14.1.28.
Lipids were extracted from hair, scalp wipings, ovarian dermoid cysts, and vernix caseosa and submitted to alkaline hydrolysis. Alumina chromatography applied to the unsaponifiable portions allowed for the separation of hydrocarbons, alcohols, and sterols. The fatty acids from the saponifiable mixtures were converted to the methyl esters and analyzed by gas chromatography in conjunction with mass spectrometry. This technique permitted the identification of the even, odd, iso, and anteiso fatty acid levels of the various pools. Analysis of the alcohols was afforded by gas chromatography of the acetates. Ratios were determined among the acidic and alcoholic components of each lipid class and the alcoholic constituents of each pool appeared to be increased by two C2-fragments over the average carbon number of the corresponding acid fraction. Branched components ranged higher in all alcohol mixtures as compared to the respective acids and thus, indicative of a specificity for the synthesis of a specificity for the synthesis of alcohols.
从头发、头皮擦拭物、卵巢皮样囊肿和胎脂中提取脂质，并进行碱性水解。将氧化铝色谱法应用于不皂化部分，可分离出烃类、醇类和甾醇。可皂化混合物中的脂肪酸转化为甲酯，并结合质谱通过气相色谱法进行分析。该技术能够鉴定各个组分中偶数、奇数、异构和反异构脂肪酸的水平。通过乙酸酯的气相色谱法对醇类进行分析。测定了每种脂质类别的酸性和醇性成分之间的比例，并且每个组分中的醇性成分似乎比相应酸组分的平均碳原子数增加了两个C2片段。与各自的酸相比，所有醇混合物中的支链成分含量更高，因此表明醇类合成具有特异性。
