Logtenberg T, Schutte M E, Inghirami G, Berman J E, Gmelig-Meyling F H, Insel R A, Knowles D M, Alt F W
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
Int Immunol. 1989;1(4):362-6. doi: 10.1093/intimm/1.4.362.
We have studied frequencies of VH gene utilization in a panel of monoclonal Epstein-Barr virus (EBV)-transformed B cell lines derived from human adult and fetal tissues as well as in monoclonal B cells obtained from fresh chronic lymphocytic leukemia (CLL) samples. The results show that IgM-secreting EBV cell lines from both fetal and adult tissues utilize VH genes from particular families roughly in proportion to estimated family size, suggesting that the repertoire of sigM-positive B cells in both fetal and adult organs is 'normalized' with respect to the V(H) gene family. In contrast, we find a highly biased pattern of VH gene expression in CLLs. The significance of these findings is discussed in the context of mechanisms that could be involved in normal B cell repertoire development and in the process of malignant transformation of precursors of CLL.
我们研究了一组源自人类成人和胎儿组织的单克隆爱泼斯坦-巴尔病毒（EBV）转化的B细胞系以及从新鲜慢性淋巴细胞白血病（CLL）样本中获得的单克隆B细胞中VH基因的利用频率。结果表明，来自胎儿和成人组织的分泌IgM的EBV细胞系利用特定家族的VH基因，其比例大致与估计的家族大小成比例，这表明胎儿和成人器官中sigM阳性B细胞的库在V(H)基因家族方面是“正常化”的。相比之下，我们发现CLL中VH基因表达存在高度偏向的模式。在可能参与正常B细胞库发育和CLL前体细胞恶性转化过程的机制背景下，讨论了这些发现的意义。
