Demaison C, David D, Letourneur F, Thèze J, Saragosti S, Zouali M
Immunogénétique cellulaire, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France.
Immunogenetics. 1995;42(5):342-52. doi: 10.1007/BF00179395.
Using CD19 B-cell selection and polymerase chain reaction-amplified cDNA libraries, we analyzed the peripheral immunoglobulin heavy chain variable repertoire of three healthy adult donors. Here we report that most of the CD19+ circulating B cells expressed unmutated VH-D-JH rearrangements. By specific VH family hybridization, we show that VH gene family utilization in the periphery roughly corresponds to the complexity of these families in the germline and appears to be relatively constant among the analyzed subjects. However, sequence data of clones picked at random from one IgM cDNA library reveals that in spite of this "random" utilization, the VH gene expression in naive circulating B cells is highly biased towards the expression of a limited set of VH genes. As previously reported by others, this restricted mechanism is also found for the D and JH segments.
利用CD19 B细胞分选和聚合酶链反应扩增的cDNA文库，我们分析了三名健康成年供体的外周免疫球蛋白重链可变区库。在此我们报告，大多数循环CD19⁺ B细胞表达未突变的VH-D-JH重排。通过特异性VH家族杂交，我们发现外周血中VH基因家族的利用情况大致与种系中这些家族的复杂性相对应，并且在所分析的个体中似乎相对恒定。然而，从一个IgM cDNA文库中随机挑选的克隆的序列数据显示，尽管存在这种“随机”利用，但幼稚循环B细胞中的VH基因表达高度偏向于有限一组VH基因的表达。正如其他人先前报道的那样，这种受限机制在D和JH区段中也有发现。
